TGEA Medium (Tryptone Glucose Yeast Extract Agar) Cat. 1190
For total count of aerobics organisms in water, dairy products, etc.
Practical information
Aplications Categories
Selective enumeration Mesophilic aerobic
Industry:Water / Dairy products
Principles and uses
TGEA Medium (Tryptone Glucose Yeast Extract Agar) is a medium used for the total count of aerobic mesophilic bacteria in water and dairy products.
Casein Peptone and Beed Extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is source of vitamins,
particularly the B-group. Dextrose is the fermentable carbohydrate providing carbon and energy. Bacteriological agar is the solidifying agent.
Formula in g/L
Glucose 1Bacteriological agar 18
Beef extract 3Casein peptone 5
Yeast extract 1
Preparation
Suspend 28 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete
dissolution. Dispense into appropriate containers and sterilize in autoclave at 121°C for 15 minutes.
Instructions for use
Pour plate technique:
- Inoculate 1 ml of the sample, (if necessary 2 continuous decimal dilutions to be able to count between 15-300 colonies per plate).
- Put 12-15 ml per plate of agar cooled to 44 - 47ºC in each Petri dish. The time of preparation shouldn’t exceed 45 minutes.
- Invert the plates and incubate at 35±2 ºC for 24 hours.
- Post incubation, count the colonies.
Quality control
Solubility Appareance Color of the dehydrated medium Color of the prepared medium Final pH (25ºC)
w/o rests Fine powder Beige Clear amber, slightly opalescent 7,0 ± 0,2
Microbiological test
Incubation conditions: (35±2 ºC / 24 h)
Microrganisms Specification
Bacillus cereus ATCC 11778 Good growth
Streptococcus agalactiae ATCC 13813 Good growth
Salmonella typhimurium ATCC 14028 Good growth
Escherichia coli ATCC 25922 Good growth
Staphylococcus aureus ATCC 25923 Good growth
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Page 1 of 2 - Revision number 1 - Date 18/06/2019
