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UTSA Microbiology Lab Midterm Review Question and answers already passed 2025, Exams of Microbiology

Newcastle UniversityMicrobiology

UTSA Microbiology Lab Midterm Review Question and answers already passed 2025

Typology: Exams

2024/2025

Available from 07/10/2025

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UTSA Microbiology Lab Midterm Review Question and answers already
passed 2025
1. Washing hands with antimicrobial
soap
is recommended before
starting your experiment,
whenever you depart from the lab,
whenever you remove your gloves
and whenever you cough onto your
hand(s).
T/F
2.
Electronic devices are permitted in
the lab, however they must remain?
3. The acronym BSL stands for
and our lab is level
.
4.
Paper towels contaminated with
micro-
bial culture should be disposed
of in the
large biohazard containers.
T/F
5. List some activities that are strictly
pro-
hibited in the microbiology
laboratory.
true
secured in you bag on the hooks
Biosafety
Laboratory
II
true
-eating
food
-wearing
shorts
-removing
and
reapplying
contact
lenses
6. Lab is open Monday-Friday during what 6 am - 9 pm
times?
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Download UTSA Microbiology Lab Midterm Review Question and answers already passed 2025 and more Exams Microbiology in PDF only on Docsity!

passed 2025

  1. Washing hands with antimicrobial soap is recommended before starting your experiment, whenever you depart from the lab, whenever you remove your gloves and whenever you cough onto your hand(s). T/F
  2. Electronic devices are permitted in the lab, however they must remain?
  3. The acronym BSL stands for and our lab is level.
  4. Paper towels contaminated with micro- bial culture should be disposed of in the large biohazard containers. T/F
  5. List some activities that are strictly pro- hibited in the microbiology laboratory. true secured in you bag on the hooks Biosafety Laboratory II true -eating food -wearing shorts -removing and reapplying contact lenses
  6. Lab is open Monday-Friday during what 6 am - 9 pm times?

passed 2025

  1. Lab is open Saturday-Sunday during what times?
  2. List any two items that are disposed of in the small biohazard containers. 6 am - 6 pm -cotton swabs - pipettes

passed 2025

  1. Agar plates are disposed of at the . large biohazard bins
  2. Test tubes are disposed of at the? rack near the edge of the bench by the sink
  3. Zinc is located where in the micro lab? reagent cabinet

passed 2025

  1. "Eyepiece," the lens the viewer looks through to see the specimen. The eye- piece usually contains a 10x power, in- volved in magnification.
  2. A rotating turret that houses the objec- tive lenses, the viewer spins the nose- piece to select different objective lenses.
  3. The lenses closest to the specimen; range in power from 4x to 100x.
  4. Specimens visible to the naked eye; power of 4x.
  5. Known as the objective lens setting for "Koehler Illumination."
  6. A process that provides optimum con- trast and resolution. ocular lenses revolving nosepiece objective lenses scanning low power; power of 10x Koehler Illumination
  7. Objective lens that is used when viewing high dry; power of 40x fungi or parasitic worms.
  8. Objective lens used when viewing bac- terial/blood smears. oil immersion; 100x

passed 2025

  1. Gathers and focuses light from the illuminator onto the specimen being viewed condenser
  2. The light source for a microscope. lamp/illuminator
  3. What does the base of the the micro- scope do?
  4. What is the function of the mechanical stage adjustment knobs?
  5. What is the function of the fine focus knob?
  6. The brings the specimen into general focus.
  7. Name the knob that controls the inten- sity of light coming from the illuminator.
  8. What part of the microscope supports the body tube? the base supports the microscope and it's where the illuminator/lamp is located they move the state up/down or left/right they fine tune the focus and increase detail of the specimen course focus knob light intensity knob the arm
  9. In bright field microscopy the specimen dark appears and the background is

passed 2025 lit

  1. In dark field microscopy the specimen is true brightly lit and the background appears dark. T/F
  2. true

passed 2025 points must be for the microscope to view them as being separate true (^) true

  1. What is the formula to calculate the limit -where D = the minimum distance at which two of resolution. points can be resolved
    • λ = wavelength of light being used -NA condenser and NA objective are the nu-

passed 2025 merical apertures of condenser lens and and objective lens

  1. Which of all the objective lenses has the oil immersion objective lens highest capacity of magnifying an ob- ject?
  2. Known as the ability of a lens to capture numerical aperture light.
  3. What is the total magnification that can be achieved by our compound micro- scope? 1000x (10x *100x)
  4. The setting up of light microscope in the Koehler Illumination very beginning to ensure that the spec- imen is well illuminated is known as?
  5. Name the WBCs that make up the gran- Neutrophils ulocytes.
  6. Name the WBCs that make up the agranulocytes.
    1. List the WBCs in order of abundance from greatest to least.

passed 2025

  1. What is the function of WBCs in the hu- man body? provide immunity against various factors such as bacterial infection, chronic inflammation, parasitic infection among other functions
  2. What are functions of neutrophils? phagocytocis and digestion of (usually) bacte- ria
  3. What are functions of eosinophils? present in inflammatory reactions (removal of antigen-antibody complexes) and destruction of multicelluar parasites (worms) with cyototox- ins and nuerotoxins
  4. What are functions of basophils? sustain inflammatory responses by releasing

passed 2025 chemicals, such as heparin and histamine.

  1. What are functions of lymphocytes? active in specific acquired immunity (as T- and B- cells). Also active as NK cells

passed 2025 neutrophils: 55% - 65% lymphocytes: 25% - 33% monocytes: 3% - 7% eosinophils: 1% - 3% basophils: <0.2% bright-field microscope condenser par-positional

passed 2025 a culture has isn't mixed with another microor- ganism, only 1 species/microorganism is pre- sent

  1. Define the term "inoculation." inoculation is a term used to describe the trans- ferring of a microbe/bacterium into a medium (broth, agar slant, or plated media)
  2. What are the five identifiers that must be labeled on each piece of experimen- tal media in this lab?
  3. Name the component that is used as solidifying agent in solid media. What is the melting point of that agent.
  4. What percentage of agar should be used in order to prepare a solid medi- um?
  5. Name 3 instruments used to transfer microorganisms in this lab.
    1. name (initials)
    2. course/section # (1061.007)
    3. experiment #
    4. date of inoculation
    5. name of organism agar; melting point = 100℃ greater than or equal to 1% -cotton swabs -inoculating needle -inoculating loop

passed 2025 What streaking technique should you use for a high density sample to isolate a microorganism?

  1. What streaking technique should you use for a low density sample to isolate a microorganism?
  2. The visible mass of cells developing from a single microorganism is known as what? zigzag streaking technique colony
  3. The acronym "CFU" stands for? colony-forming unit
  4. After inoculation of your tubes and plates for exercise 1-4 (Common Aseptic Transfers and In- oculation Methods) and exercise 1-5 (Streak Plate Methods of Isolation) you incubated them at °C for - hours.
  5. How many drops of E. coli and Serretia marcescens did you mix to prepare the mixed culture and perform streaking for exercise 1- (Streak Plate Method of Iso- lation)?
    1. A word that describes transferring mi- crobes from their pure culture to a ster- ile medium without contamination of yourself, others, the environment, the

passed 2025 37°C 24-48 hours E. coli: 2 Serretia marcescens: 10 aseptically