Download UTRGV Gen. Microbio Lab Midterm (Schuenzel) Question and answers correctly solved 2025 and more Exams Microbiology in PDF only on Docsity!
UTRGV Gen. Microbio Lab Midterm
(Schuenzel) Question and answers
correctly solved 2025
What container would you put a used coverslip in? - correct answers broken glass container Which container would you place your used gloves in? - correct answers biohazardous waste Which container would you put a razor in? - correct answers sharps container Which piece of glassware has an equal circumference until the top where the opening has a much smaller circumference? - correct answers media bottle Which piece of glassware has a progressively smaller circumference from the bottom to the top? - correct answers Erlenmeyer flask Which machine is used to heat a mixture and stir a mixture using electromagnetic force? - correct answers hot plate and magnetic stirrer Which piece of equipment is used to sterilize equipment and media? - correct answers autoclave Which piece of equipment is used to avoid contamination when working with microbes? - correct answers laminar flow hood
Which piece of equipment measures the amount of material based on absorbance? - correct answers spectrophotometer Which machine is used to perform polymerase chain reactions? - correct answers thermocycler/PCR machine Which machine is used to keep liquids at a constant temperature? - correct answers waterbath Which machine is used to grow and maintain microorganisms at optimal physical conditions? - correct answers incubator Which tool is used to move microorganisms between cultures and can be repeatedly sterilized? - correct answers inoculating loop Which machine agitates a mixture in a test tube to make a homogenized mixture? - correct answers vortex mixer Which machine rotates a mixture to separate out the different parts? - correct answers centrifuge Which machine is used to measure masses? - correct answers analytical balance Which piece of glassware is used to move liquid culture around an agar plate evenly? - correct answers spreader Which piece of glassware is the most precise way to measure large amounts of liquid? - correct answers graduated cylinder
The most common type of microscope used - correct answers brightfield microscope microscope that causes the light source to hit the specimen at a severely oblique angle - correct answers darkfield microscope microscope that can clearly view live organisms without stains - correct answers phase contrast microscope When using the scanning objective, the objective magnification would be - correct answers 4 When using the low power objective, the objective magnification would be - correct answers 10 When using the high power objective, the objective magnification would be - correct answers 40 When using the oil immersion objective, the objective magnification would be - correct answers 100 What type of cell wall (gram negative/positive) was E. coli? - correct answers gram negative Which type of cell wall (gram negative/positive) was P. aeruginosa? - correct answers gram negative Which type of cell wall (gram negative/positive) was B. subtilis? - correct answers gram positive
Which type of cell was (gram negative/positive) S. epidermis? - correct answers gram positive Which supergroup do Kinetoplastida, Diplomonads, Pabasilids, Euglenazoans, and Euglenids belong to? - correct answers Excavata Which supergroup do Cercozoa, Radiolarians, and Foraminiderans belong to?
- correct answers Rhizaria Which supergroup do Apicoplexans, Ciliates, and Dinoflagellata belong to? - correct answers Alveolata The phylum Nematoda belongs to which kingdom? - correct answers Animal The phylum Platyhelminthes belongs to which kingdom? - correct answers Animal Which fungal phylum contains bread molds? - correct answers Zygomycota Which phylum contains the fungus that produces penicillin and brewer's yeast (Saccharomyces)? - correct answers Ascomycota Which phylum contains the plant pathogenic fungi known as smuts and rusts?
- correct answers Basidiomycota Which virus classification includes double stranded DNA? - correct answers Class I
Which type of stain uses a single dye? - correct answers simple stain Which type of stain uses 2 dyes - a primary dye and a counterstain? - correct answers differential stain What cell structure does gram staining react to? - correct answers cell wall What waxy material in the cell wall does acid-fast staining react to? - correct answers mycolic acid What cell structure gets stained when the cell in under environmental stress? - correct answers spore Name the 2 stains used in Gram staining - correct answers crystal violet and safranin Name the 2 stains used in acid-fast staining - correct answers fuchsin and methylene blue Name the 2 stains used in spore staining - correct answers malachite green and safranin Name the one stain that can be used in negative staining - correct answers nigrosin Which type of cell wall is indicated by purple/blue in Gram staining? - correct answers gram positive
Which type of cell wall indicated by pink in Gram staining? - correct answers gram negative With spore staining, green indicates which structure? - correct answers spore With spore staining, red indicates what type of cell? - correct answers vegetative With acid-fast staining, Mycobacterium (acid-fast bacteria) will stain which color because of the presence of mycolic acid? - correct answers red With acid-fast staining, non-acid-fast bacteria will stain which color? - correct answers blue Is Staphylococcus aureus gram positive or negative? - correct answers gram positive Is Pseudomonas aeruginosa gram positive or gram negative? - correct answers gram negative Is Bacillus megaterium gram positive or gram negative? - correct answers gram positive Is Moraxella catarrhalis gram positive or negative? - correct answers gram negative Does the bacteria Clostridium difficile produce spores (yes or no)? - correct answers yes
This type of media is used to count the number of microbes in agricultural, industrial, or environmental samples - correct answers Enumeration This type of media is used to test the effectiveness of antimicrobial drugs, disinfectants, and antiseptics - correct answers Assay This type of media contain pure chemical nutrients that vary little from 1 source to another and have a molecular content with an exact formula - correct answers Synthetic media This type of media is not chemically definable by an exact formula - correct answers Nonsynthetic media This type of media has large molecules such as proteins, polysaccharides, and lipids that can vary greatly in exact composition - correct answers complex media Nutrient agar or nutrient broth is a general purpose media used for which groups? - correct answers bacteria and fungi Potato dextrose agar is a general purpose media used for which groups? - correct answers fungi Trypticase soy agar or broth is a general purpose media used for which groups? - correct answers bacteria NA stands for what? - correct answers Nutrient Agar
NB stands for what? - correct answers Nutrient Broth PDA stands for what? - correct answers Potato Dextrose Agar TSA stands for what? - correct answers Trypticase Soy Agar MSA stands for what? - correct answers Mannitol Salt Agar EMB stands for what? - correct answers Eosin Methylene Blue MCA stands for what? - correct answers MacConkey's Agar What is commonly used to culture algae? - correct answers salt agar What is commonly used to culture paramecium? - correct answers Hay infusion What is commonly used to culture viruses? - correct answers live host cells The selective and differential media used to isolate Staphylococcus - correct answers MSA The selective and differential media used to isolate enteric bacteria that hydrolyze urea - correct answers EMB this selective and differential media is used to isolate enteric bacteria that ferment lactose - correct answers MCA
cell division where genome replication is followed directly by the physical separation into 2 new, identical cells - correct answers binary fission cell division where genome replication is separated by a gap phase from the physical separation in 2 new, identical cells - correct answers mitosis In this phase, limiting factors intensify becoming detrimental to any cell growth
- correct answers death phase in this phase, the cells are adjusting to the environment - correct answers lag phase in this phase, the cells reach the maximum rate of cell division - correct answers log phase in this phase, cells will stop growing or grow very slowly - correct answers stationary phase in this phase, the majority of the cells are alive, but dead cells start to accumulate - correct answers stationary phase in this phase, there are many living cells and few to none dead cells - correct answers log phase in this phase, there are few cells present - correct answers lag phase in this phase, the dead cells take up a larger portion of the culture and little to no growth occurs - correct answers death phase
Of the following absorbance reading samples, which one has more cells present? a). b). c) .85 - correct answers c Of the following absorbance reading samples, which one has the fewest cells present? a). b). c) .19 - correct answers a How many cells are present after 9 generations (starting from 1 cell) of exponential growth (2^n)? - correct answers 512 How many cells are present after 13 generations (starting from 1 cell) of exponential growth (2^n)? - correct answers 8192 A dilution of 1 mL bacteria from broth into 1 mL broth or water is what dilution ratio? - correct answers 1: A dilution of 1 mL bacteria from broth into 3 mL broth or water is what dilution ratio? - correct answers 1: A serial dilution of 1:10 followed by 1:100 followed by 1:100 gives a final dilution of - correct answers 1: A serial dilution of 1:10 followed by 1:10 followed by a 1:100 gives a final dilution of - correct answers 1:
A 1:1,000,000 is what dilution factor? (Write the whole number, i.e. -2,-3) - correct answers - A 1:100,000 is what dilution factor? (Write the whole number, i.e. -1,-2) - correct answers - A 1:100,000,000 is what dilution factor? (Write the whole number, i.e. -1,-2) - correct answers - A 1:10,000,000 is what dilution factor? (Write the whole number, i.e. -4,-5) - correct answers -
