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This is a lab report which talks about The Effect of Chemicals on the Pulse Rate of Blackworms.
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Name: Vy Pham Instructor Lab: Gurneet Bola Shah Class: BIOL Lab – S04 Date: October 14, 2020 The Effect of Chemicals on the Pulse Rate of Blackworms (Lumbriculus variegatus ) Activity The pulse rate of blackworms ( Lumbriculus variegatus ) increased as the concentration of caffeine increased (Figure 1). The mean pulse rate of the control group (no caffeine) was lowest, at 13.5 beats per minute ± 1.3 (95% C.I.). The mean pulse rate increased in the treatment group #1 (low concentration of caffeine), at 16.2 beats per minute ± 1.6 (95% C.I.). The highest mean pulse rate was 20.1 beats per minute ± 2.7 (95% C.I.), that was the pulse rate of the treatment group #2 (high concentration of caffeine). Figure 1. Mean pulse rate (beats per minutes) of blackworms ( Lumbriculus variegatus ) at no caffeine (control), low concentration of caffeine and high concentration of caffeine. Error bars represent 95% confidence intervals of the mean (n=10).
The objective of this experiment was to determine whether the pulse rate of blackworms was affected by the chemical (caffeine) with different concentrations of caffeine. The results of our experiment allow us to reject the null hypothesis because the 95% confidence interval of the control group (no caffeine) does not overlap with the confidence intervals of the treatment groups ( low and high concentration of caffeine). We provide support for the alternative hypothesis, which states the concentration of caffeine will have effect on the pulse rate (beats per minute) of black worms ( Lumbriculus variegatus ). Thus, we can conclude that caffeine has an effect on the pulse rate in blackworms (( Lumbriculus variegatus). One main source of error in this experiment was that the sample of blackworms chosen was different in the age and the size, that also can affect the pulse rate of worms. The differences of size and age of blackworms make the effect of caffeine on them not be similar to them. This error can be fixed with choosing the sample blackworms with same age and size. In additional, error can occur during counting the pulse rate by looking at different segments of blackworms. This make the pulse rate can be higher or lower. We can avoid this by making sure we are looking at the same segment of blackworm during counting pulse rate even if they are moving. Future studies may benefit from studying the effect of the chemical which makes the pulse rate of blackworms decrease such as beta-blockers and compare with the chemical has opposite effect. For example, we can make the experiment to compare the effect of propranolol and caffeine on the pulse rate of blackworms with same age and size. In conclusion, we expected the concentration of caffeine has an impact on the pulse rate of blackworms, with higher concentration of caffeine has larger effect on the pulse rate. Our