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Saccharomyces - Applied Molecular Biology - Exam, Exams of Cellular and Molecular Biology

Sri Ramachandra Medical College and Research InstituteCellular and Molecular Biology

Saccharomyces, Feature of Agrobacterium Tumefaciens, Plant Meristem, Selectable Markers, Deriving Transgenic Plants, Haemophilus Influenzae, Arabidopsis Thaliana, Rna Polymerase Catalyses, Synthesis of Mrna are some points from questions of Applied Molecular Biology subject.

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ARHOLIADAU EXAMINATIONS
SEMESTER 1 EXAMINATIONS 2010
IBERS (BIOLOGICAL SCIENCES)
BS23720 APPLIED MOLECULAR BIOLOGY
TIME ALLOWED: THREE HOURS
ANSWER ALL QUESTIONS FROM SECTION A IN THE SPACE PROVIDED ON
THE QUESTION PAPER, AND TWO QUESTIONS FROM SECTION B IN THE
SEPARATE ANSWER BOOKS
Rhif Cyfeirio’r Myfyriwr/
Student Reference Number:
……………………………………………
Dyddiad/Date
………………………………………………
Ystafell Arholiad/Examination Room
………………………………………………
Rhif y Sedd/Seat Number
………………………………………………
1. Please give the information requested above
and enter your name in the space provided at
the top right of this page. If your answers are
to be marked under a scheme approved by
the College whereby the examiners will not
know your identity, fold over and seal down
the top right-hand corner (using the gummed
slip provided) in order to conceal your name.
2. The answers must be legibly written. The
Examiners can either ignore illegible script or
have it typed and the expense of typing
charged to the candidate.
3. Write your answers in ink.
4. This paper must be handed in complete.
5. If you leave the examination early, please do
so quietly so as not to disturb other
candidates.
1. Rhowch y wybodaeth angenrheidiol yn y blychau
uchod a rhowch eich enw yn y man gwag ar dop y
tudalen ar y dde. Plygwch a seliwch y cornel ochr
dde ar dop y tudalen (gan ddefnyddio’r slip gludiog
a ddarperir) er mwyn cuddio eich enw.
2. Rhaid i’r atebion fod wedi eu hysgrifennu’n glir.
Gall yr Arholwyr nail ai anwybyddu sgript
annealladwy, neu drefnu i’w theipio a chael yr
ymgeisydd i gwrdd â chost y gwaith.
3. Ysgrifennwch eich atebion mewn inc.
4. Ni ddylid torri allan unrhyw ran o’r llyfr hwn; rhaid ei
ddychwelyd mewn ffurf gyflawn.
5. Os ymadewch â’r ystafell arholi yn gynnar,
gwnewch hynny’n dawel os gwelwch yn dda rhag
aflonyddu ar ymgeiswyr eraill.
Fold over and stick down/plygwch y gornel drosodd
Cyfenw/Surname .............................................
Enwau Cyntaf/
Forenames ......................................................
........................................................................
pf3
pf4
pf5

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ARHOLIADAU EXAMINATIONS

SEMESTER 1 EXAMINATIONS 2010

IBERS (BIOLOGICAL SCIENCES)

BS23720 APPLIED MOLECULAR BIOLOGY

TIME ALLOWED: THREE HOURS

ANSWER ALL QUESTIONS FROM SECTION A IN THE SPACE PROVIDED ON

THE QUESTION PAPER, AND TWO QUESTIONS FROM SECTION B IN THE

SEPARATE ANSWER BOOKS

Rhif Cyfeirio’r Myfyriwr/ Student Reference Number: ……………………………………………

Dyddiad/Date ………………………………………………

Ystafell Arholiad/Examination Room ………………………………………………

Rhif y Sedd/Seat Number ………………………………………………

  1. Please give the information requested above and enter your name in the space provided at the top right of this page. If your answers are to be marked under a scheme approved by the College whereby the examiners will not know your identity, fold over and seal down the top right-hand corner (using the gummed slip provided) in order to conceal your name.
  2. The answers must be legibly written. The Examiners can either ignore illegible script or have it typed and the expense of typing charged to the candidate.
  3. Write your answers in ink.
  4. This paper must be handed in complete.
  5. If you leave the examination early, please do so quietly so as not to disturb other candidates. 1. Rhowch y wybodaeth angenrheidiol yn y blychau uchod a rhowch eich enw yn y man gwag ar dop y tudalen ar y dde. Plygwch a seliwch y cornel ochr dde ar dop y tudalen (gan ddefnyddio’r slip gludiog a ddarperir) er mwyn cuddio eich enw. 2. Rhaid i’r atebion fod wedi eu hysgrifennu’n glir. Gall yr Arholwyr nail ai anwybyddu sgript annealladwy, neu drefnu i’w theipio a chael yr ymgeisydd i gwrdd â chost y gwaith. 3. Ysgrifennwch eich atebion mewn inc. 4. Ni ddylid torri allan unrhyw ran o’r llyfr hwn; rhaid ei ddychwelyd mewn ffurf gyflawn. 5. Os ymadewch â’r ystafell arholi yn gynnar, gwnewch hynny’n dawel os gwelwch yn dda rhag aflonyddu ar ymgeiswyr eraill.

Fold over and stick down/plygwch y gornel drosodd

Cyfenw/Surname .............................................

Enwau Cyntaf/ Forenames ......................................................

SECTION A (50 marks) ANSWER ALL QUESTIONS

TICK THE APPROPRIATE BOX WHERE NECESSARY, e.g []

  1. Which is the correct statement? Shine Dalgarno sequence(s) ( 1 mark ) a. [ ] are essential for mRNA stability. b. [ ] directly aid the binding of 60S ribosome. c. [ ] are pyrimidine rich. d. [ ] can base pair with the 16S rRNA in ribosomes. e. [ ] none of the above.
  2. What is the definition of Tm? Give a simple equation to calculate the Tm of a primer.( 2 marks )
  3. Which of the following statements is correct concerning Saccharomyces cerevisiae? ( 1 mark ) a. [ ] It was the first multicellular eukaryote sequenced. b. [ ] It has the same number of genes as the mouse. c. [ ] It was the first organism in which RNAi was discovered. d. [ ] It is ideal for studying apoptosis. e. [ ] It was the first eukaryote sequenced.
  4. The herbicide glyphosate kills plants by ( 1 mark ): a. [ ] inhibiting rubisco b. [ ] uncoupling photosystem 1 c. [ ] inhibiting an enzyme of the chloroplast d. [ ] preventing translocation of nutrients e. [ ] its cytotoxicity.
  5. What is the fundamental difference between ordered clone sequencing and shotgun sequencing of genomes? ( 2 marks )
  6. What property of nucleic acids is exploited in the method of in situ hybridisation? ( 2 marks )
  1. Look at the schematic of a Ti plant plasmid shown below What is encoded by the
    1. tms1, tms2 genes ( 1 mark )
    2. tmr gene ( 1 mark )
    3. ocs gene ( 1 mark )
    4. What is the function of the border sequences ( 1 mark )
    5. Give two roles for the vir operon genes ( 2 mark )
  2. What is cDNA and how is it generated? ( 2 marks )
  3. Describe the principles behind inverse PCR and explain why it is used ( 2 marks )
  1. Briefly describe how was the genome of Haemophilus influenzae was sequenced? ( 5 marks )
  2. Delete the incorrect bracketed words from the statement below: ( 4 marks )

Increased cereal yields in the twentieth century resulted from breeding of [taller][shorter] crops. In wheat, the major genes involved were [dominant][recessive], and were isolated in the 1990s via [transformation][transposon tagging] of the orthologous gene in [yeast][ Arabidopsis thaliana ].

  1. Briefly describe what microinjection is and how it works ( 2 marks )
  2. In the E. coli chromosome there is/are ( 1 mark ) a. [ ] a single origin of replication b. [ ] 10 origins of replication c. [ ] 100 origins of replication d. [ ] 1,000 origins of replication e. [ ] 10,000 origins of replication.
  3. Sigma factors are important for what process? ( 1mark )
  4. How far is the Pribnow box (TATAAT) present in prokaryotic promoters from the start of transcription? ( 1 mark )
  5. List the different sites present in a ribosome that tRNA passes through ( 3 marks )
  6. Which eukaryotic RNA polymerase catalyses the synthesis of mRNA? ( 1 mark )

SECTION B (50 marks)

ANSWER TWO QUESTIONS EACH IN A SEPARATE ANSWER BOOK

  1. Describe the role of the model species Arabidopsis thaliana in the molecular genetic isolation of the semi-dwarfing gene responsible for the ‘Green Revolution’ in wheat agriculture. ( 25 marks )
  2. Discuss how plants can be transformed by biolistic bombardment? ( 25 marks )
  3. Explain how antisense technology and the binary vector system were used together to produce a tomato with delayed ripening. ( 25 marks )
  4. Compare and contrast the initiation of protein synthesis in eukaryotes with that in prokaryotes. ( 25 marks )
  5. Describe the major principles concerned with the use of bacteriophage vectors to generate libraries of genomic DNA. ( 25 marks )
  6. With the use of diagrams when appropriate, provide a brief account of two of the following ( 12.5 marks per question part )

a) What is blue-white screening? Describe the principles behind this screening procedure.

b) The procedures involved in DNA gel blot (Southern) analysis

c) PCR and RT_PCR

END