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PCBI105L Microbiology and Parasitology LAB M4 Assignment 1 Preparing Serial Dilution at Home
Typology: Exercises
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May 2X, 202X
Prepare the following materials for this exercise: ● 7 glass vials, cups, or bottles or any suitable transparent container ● Measuring instrument (Syringe, measuring cups, measuring spoons, etc.) ● Any colored liquid of your choice (this is equivalent to your specimen) ● Water PROCEDURES: Perform the following procedures step-by-step: Remember that we will be following the 9:1 principle, wherein there will be 9 parts water and 1 part "specimen".
Volume pipetted Total volume Sample volume : total volume (ratio) Individual dilution factor Total dilution factor Tube #1 10ml 100ml 1:10 1/10 1/ Tube #2 10ml 100ml 1:10 1/10 1/ Tube #3 10ml 100ml 1:10 1/10 1/1, Tube #4 10ml 100ml 1:10 1/10 1/10, Tube #5 10ml 100ml 1:10 1/10 1/100, Tube #6 10ml 100ml 1:10 1/10 1/1,000, Tube #7 10ml 100ml 1:10 1/10 1/10,000,