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Microbiology Lab Midterm Troy University Question and answers already passed 2025, Exams of Microbiology

Microbiology Lab Midterm Troy University Question and answers already passed 2025

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2024/2025

Available from 07/10/2025

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Microbiology Lab Midterm Troy
University Question and answers
already passed 2025
ubiquitous - correct answers microbes can be found anywhere you look for
them
normal flora - correct answers microbes usually associated with animal body
tissue
nosocomial infections - correct answers infection originate in hospital
environment
colony - correct answers visible cluster of bacteria growing on the surface of
or within a solid medium, presumably cultured from a single cell
colony formation - correct answers when one microbe is deposited on an agar
surface, nutrients are provided to the cell and begins to divide to form a colony
sterile - correct answers free of any living organism
contamination - correct answers introduction of any unwanted microbes
why is the agar plate placed face up? - correct answers if condensation drips
onto the agar gel with the sample, it will cause the bacteria to grow all over the
plate
agar plates are used for - correct answers isolate organism from mixed culture
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Microbiology Lab Midterm Troy

University Question and answers

already passed 2025

ubiquitous - correct answers microbes can be found anywhere you look for them normal flora - correct answers microbes usually associated with animal body tissue nosocomial infections - correct answers infection originate in hospital environment colony - correct answers visible cluster of bacteria growing on the surface of or within a solid medium, presumably cultured from a single cell colony formation - correct answers when one microbe is deposited on an agar surface, nutrients are provided to the cell and begins to divide to form a colony sterile - correct answers free of any living organism contamination - correct answers introduction of any unwanted microbes why is the agar plate placed face up? - correct answers if condensation drips onto the agar gel with the sample, it will cause the bacteria to grow all over the plate agar plates are used for - correct answers isolate organism from mixed culture

agar slants are used for - correct answers storage agar deeps are used for - correct answers testing oxygen requirements/motility liquid broths are used for - correct answers produce large amounts of bacteria aseptic technique - correct answers ability to transfer bacterial cultures (cells) from one place to another without contaminating the original culture, the new medium, or the environment. what is the purpose of the quadrant streak plate method of isolation? - correct answers to obtain isolated colonies from a mixed culture of bacteria light microscopy - correct answers images viewed under the light microscope are reversed (mirror image) and inverted (upside down) magnification - correct answers how much bigger a sample appears to be under the microscope than it is in real life resolution - correct answers the ability to distinguish between 2 points on an image (the amount of detail) total magnification = - correct answers ocular lens power X objective lens power describe a simple stain - correct answers -basic stain -methylene blue

selective media - correct answers selectively inhibit/foster growth differential media - correct answers indicator dye to distinguish defined media - correct answers exact chemical composition known complex media - correct answers easy to use Mannitol Salt Agar (MSA) - correct answers -high salt Mannitol + phenol red (indicator) -good growth = organism is not inhibited by NaCl -poor/no growth = organism is inhibited by NaCl -Coagulase positive = yellow colonies produced -Coagulase negative = small pink or red colonies with no color change to the medium Phenyl-ethyl Alcohol (PEA) agar - correct answers -phenylethyl alcohol -inhibits or reduces growth of GRAM NEGATIVE by interfering with DNA synthesis Eosin Methylene Blue (EMB) agar - correct answers -Osin Y + methylene blue -inhibits or reduces growth of GRAM POSITIVE -if lactose is fermented (color change) acid was produced and there will be a color change -used to isolate fecal coliform coliform - correct answers type of bacteria that lives in the intestines

MacConkey Agar (MAC) - correct answers -Lactose and neutral red (indicator dye)

  • Red= acid production (from fermented lactose) -inhibits growth of most GRAM POSITIVE -Crystal Violet dye (optional) -inhibits growth of GRAM POSITIVE free living - correct answers organisms that do not reside on or in a particular plant or animal host reservoir - correct answers any area including areas outside the typical host organism where a microbe resides and serves as a potential source of infection dark field microscopy - correct answers a special condenser is used so only the light reflected off of the specimen enters the objective what does heat fixing do - correct answers -kills most of the bacteria, -makes them adhere to the slide, -coagulates cytoplasmic proteins to make them more visible -distorts the cells to some extent