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Microbiology Lab Midterm Troy University Question and answers already passed 2025
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ubiquitous - correct answers microbes can be found anywhere you look for them normal flora - correct answers microbes usually associated with animal body tissue nosocomial infections - correct answers infection originate in hospital environment colony - correct answers visible cluster of bacteria growing on the surface of or within a solid medium, presumably cultured from a single cell colony formation - correct answers when one microbe is deposited on an agar surface, nutrients are provided to the cell and begins to divide to form a colony sterile - correct answers free of any living organism contamination - correct answers introduction of any unwanted microbes why is the agar plate placed face up? - correct answers if condensation drips onto the agar gel with the sample, it will cause the bacteria to grow all over the plate agar plates are used for - correct answers isolate organism from mixed culture
agar slants are used for - correct answers storage agar deeps are used for - correct answers testing oxygen requirements/motility liquid broths are used for - correct answers produce large amounts of bacteria aseptic technique - correct answers ability to transfer bacterial cultures (cells) from one place to another without contaminating the original culture, the new medium, or the environment. what is the purpose of the quadrant streak plate method of isolation? - correct answers to obtain isolated colonies from a mixed culture of bacteria light microscopy - correct answers images viewed under the light microscope are reversed (mirror image) and inverted (upside down) magnification - correct answers how much bigger a sample appears to be under the microscope than it is in real life resolution - correct answers the ability to distinguish between 2 points on an image (the amount of detail) total magnification = - correct answers ocular lens power X objective lens power describe a simple stain - correct answers -basic stain -methylene blue
selective media - correct answers selectively inhibit/foster growth differential media - correct answers indicator dye to distinguish defined media - correct answers exact chemical composition known complex media - correct answers easy to use Mannitol Salt Agar (MSA) - correct answers -high salt Mannitol + phenol red (indicator) -good growth = organism is not inhibited by NaCl -poor/no growth = organism is inhibited by NaCl -Coagulase positive = yellow colonies produced -Coagulase negative = small pink or red colonies with no color change to the medium Phenyl-ethyl Alcohol (PEA) agar - correct answers -phenylethyl alcohol -inhibits or reduces growth of GRAM NEGATIVE by interfering with DNA synthesis Eosin Methylene Blue (EMB) agar - correct answers -Osin Y + methylene blue -inhibits or reduces growth of GRAM POSITIVE -if lactose is fermented (color change) acid was produced and there will be a color change -used to isolate fecal coliform coliform - correct answers type of bacteria that lives in the intestines
MacConkey Agar (MAC) - correct answers -Lactose and neutral red (indicator dye)