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Microbiology Lab - Midterm Study Guide Question and answers
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1. List the 5 I's
1.
inoculation
2.
incubation
3.
isolation
4.
inspection
5.
identification
2. What does inoculation involve?
The
implantation
of
microorganisms
into
or upon a culture media. Often, it involves
spreading the sample on the surface of
a solid medium or intruding the sample
into a flask or tube filled with liquid media.
3. What does incubation create?
It
creates
the
proper
growth
temperature
and other conditions. Promotes multipli-
cation of the microbes over a period of
time.
4. What does incubation produce?
A
culture.
The
visible
growth
of
the
mi-
crobe in or on the medium.
5.
What is isolation?
It is a result of inoculation and incuba-
tion. Isolated microbes may take the form
of separate colonies (discrete mounds
of cells) on solid media, or turbidity
(free floating cells) in liquid broths.
6. What is done during inspection?
Colonies
or
broth
cultures
are
observed
