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Microbiology lab midterm Question and answers already passed 2025, Exams of Microbiology

Microbiology lab midterm Question and answers already passed 2025

Typology: Exams

2024/2025

Available from 07/10/2025

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Microbiology lab midterm Question and answers already passed
2025
1. A disinfectant is used on your work surface
Before
the
beginnig
of
lab
procedures
After
all
work
is
compete
After
any
spill
of
live
mi-
croorgnisms
2. Which biosafety levels do most introductory
microbi-
ology students work with?
BSL-
1
3.
Which of the following should be worn at ALL times in
Lab coat
lab?
Closed
toed
shoes
4. Watches, bracelets, and rings should be removed pri- True
or to washing hands
5. Using a cell phone in lab is permissible since it can be False
used for timing experiments
6. Which of the following would be most appropriate for Biohazard bag or
bin
disposal of cultures on agar plates?
7. How long should hands be lathered while washing?
20
seconds
8. In which circumstance is food/or beverage allowed in Never
the lab?
9. You may eat a snack in lab as long as there are no bac-
False
terial cultures in the bench and you have
pf3
pf4
pf5
pf8
pf9
pfa

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  1. A disinfectant is used on your work surface Before the beginnig of lab procedures After all work is compete After any spill of live mi- croorgnisms
  2. Which biosafety levels do most introductory microbi- ology students work with?

BSL- 1

  1. Which of the following should be worn at ALL times in Lab coat lab? Closed toed shoes
  2. Watches, bracelets, and rings should be removed pri- True or to washing hands
  3. Using a cell phone in lab is permissible since it can be False used for timing experiments
  4. Which of the following would be most appropriate for Biohazard bag or bin disposal of cultures on agar plates?
  5. How long should hands be lathered while washing? 20 seconds
  6. In which circumstance is food/or beverage allowed in Never the lab?
  7. You may eat a snack in lab as long as there are no bac- False terial cultures in the bench and you have

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thoroughly washed your hands

  1. Jackie went to the sink at the end of lab to wash her hands. After removing her watch and rings, she turned on the water, wet her hands, added soap and thoroughly rubbed and lathered her hands for 30 sec- Jackie should have used pa- per towels to turn ott the faucet.

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Personal protective equip- ment

  1. Infectious lab waste is sterilized prior to disposal by Autoclaving (steam auto- clave)
  2. sharps container

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Where should glass slides, pipettes, and capillary tubes be disposed?

  1. Which items can be discarded in a regular trash can? Uncontaminated waste pa- per Wrappers from swabs or from disposable pipettes Used glass slides
  2. In science experiments if my hypothesis is not proven False as I expected, it's OK to change it after I see the results obtained
  3. Maria wants to know if the amount of sunlight in a garden affects the tomato size? To answer this ques- tion, Maria has 2 plants growing at the same time. Plant #1 is exposed to more light than plant #2. In this example, the measurement of size of the tomatoes is called?
  4. Maria wants to know if the amount of sunlight in a garden affects the tomato size? To answer this ques- tion, Maria has 2 plants at the same time. Plant #1 is exposed to more light than plant #2. For experiment, when Maria measures the size of the tomato after growing it for 10 days, that is called:
    1. In order to be called a theory, a scientific idea has to undergo a great deal of testing

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is exposed to more light than plant #2. In this exam- ple, the amount of sunlight is called?

  1. Which of the following objectives should be used with 100x objective immersion oil?
  2. Put the lenses in the order they should be used when 1. Low power lenses viewing a specimen with a brightfield microscope
    1. High dry lens
    2. Oil immersion lens
  3. Which of the following objective magnifications is best 100x objective for observing bacteria?
  4. The coarse and fine a focus knobs adjust the distance The stage and the objective between:
  5. Only lint-free, optically safe tissue should be used to wipe of microscope lenses lens True
  6. The coarse focus knob can be used to adjust the focus False when using any of the objective lenses
  7. While using a lab photo atlas, photo is listed as having The 40x objective a total magnification of 400x. Which of the following objectives was used in order to take the photo with a microscope that had 10x oculars?
  8. Compound microscopes are able to maintain proper focus

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of a specimen when moving from Low power to higher power magnification due to a property known as

  1. In the lab, you are using a binocular compound light microscope with 10x oculars. Match the objective for Parfocal
    1. 40x objective
    2. 100x objective with im-

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  1. Petri dishes should be incubated with the lid side up False
  2. Which term refers to the introduction of unwanted microorganisms? contamination

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  1. The goal of techniques is to minimized the contamination of broth original and newly inoculated cultures as well as to protect the lab worker from contamination Aseptic
  2. To retrieve a sample from a culture tube with an inoc- Removed and held with the ulating loop, the cap of the tube is fingers of the loop hand
  3. Observation of broth after transfer can show whether False or not contamination has occurred
  4. A substance used to support the growth of microbial life is referred to as a Culture medium