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Microbiology Lab Midterm Barbosa Question and answers correctly solved 2025, Exams of Microbiology

Microbiology Lab Midterm Barbosa Question and answers correctly solved 2025

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2024/2025

Available from 07/10/2025

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Microbiology Lab Midterm Barbosa Question and answers correctly
solved 2025
1.
parfocal
only a little fine adjustment is needed to bring a specimen
into
focus when switching from low to high power
2.
iris
diaphragm
used to vary the illumination of the object being viewed
3.
rheostat
changes the intensity of the light beam
4.
aseptic
technique
prevent the contamination of the culture and spreading of
microor-
ganisms into
the
environment
5. How can bacterial culture
media be distinguished?
composition, consistency, and purpose
6.
Advantages
of
Solid
Media
allows for isolation of bacteria as pure culture; mixed bacteria
can
be
separated
7.
agar
bacteriologically
inert
8.
semisolid
media
useful for the cultivation of microaerophilic bacteria or
determina-
tion of bacterial motility
9.
all
purpose
media
TSA
(tryptic
soy
agar)
and
TSB
(tryptic
soy
broth)
10.
turbid
dittusion
uniform
throughout
the
broth
11.
sediment
settles
at
the
bottom
12.
pellicle
flocculent
growth,
some
may
float
to
the
top
13.
endospores
not readily killed by many antimicrobial treatments (may be
central,
terminal, or subterminal)
pf3
pf4
pf5

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solved 2025

  1. parfocal only a little fine adjustment is needed to bring a specimen into focus when switching from low to high power
  2. iris diaphragm used to vary the illumination of the object being viewed
  3. rheostat changes the intensity of the light beam
  4. aseptic technique prevent the contamination of the culture and spreading of microor- ganisms into the environment
  5. How can bacterial culture media be distinguished? composition, consistency, and purpose
  6. Advantages of Solid Media allows for isolation of bacteria as pure culture; mixed bacteria can be separated
  7. agar bacteriologically inert
  8. semisolid media useful for the cultivation of microaerophilic bacteria or determina- tion of bacterial motility
  9. all purpose media TSA (tryptic soy agar) and TSB (tryptic soy broth)
  10. turbid dittusion uniform throughout the broth
  11. sediment settles at the bottom
  12. pellicle flocculent growth, some may float to the top
  13. endospores not readily killed by many antimicrobial treatments (may be central, terminal, or subterminal)

solved 2025

  1. germination not a reproductive process, but a process of ditterentiation
  2. gram stain

solved 2025

  1. Voges-Proskauer detects acetoin, Barritt's reagents positive: red (E. aerogenes) negative: yellow (E. coli)

solved 2025

  1. Citrate Utilization Simmons citrate (sodium citrate as carbon source, ammonium salt as nitrogen, bromothymol blue as pH indicator) positive: turns royal blue, uses citrate (E. aerogenes) negative: stays green (E. coli)
  2. Gelatinase Test positive: media was liquefied (B. subtilis) negative: no change (E. coli)
  3. deamination the removal of an amino group
  4. Phenylalanine deamination of phenylalanine can be detected using ferric iron (carboxylic acid produced by breakdown turns green) positive: green, organic acids present (P. vulgaris) negative: no change, no organic acids (E. coli)
  5. Urease Test breakdown of urea to form ammonia, phenol red turns pink when alkaline positive: bright pink (P. vulgaris) negative: orange (E. coli)
  6. selective media special chemicals are added that allow certain types of organisms to grow while inhibiting the growth of others
  7. differential media used to ditterentiate closely related organisms or groups of organ- isms

solved 2025 ative appears colorless; determined by neutral red (S. enteriditis, E. coli)

  1. MSA (mannitol salt) agar selective and ditterential used for isolation of staphylococci and high salt concentration inhibits growth other than staphylococci (S. aureus changes the indicator to yellow) (S. epidermis is a non mannitol fermenter no color change)
  2. blood agar ditterential, provides growth factors required by fastidious pathogens (Streptococcus species)
  3. fastidious any organism that has a complex nutritional requirement, will only grow when specific nutrients are included in it's diet
  4. hemolysis destruction of erythrocytes
  5. beta hemolysis complete breakdown of red blood cells, complete zone of clearing
  6. alpha hemolysis partial destruction of RBCs and produces a greenish discoloration of the agar around the colonies
  7. gamma hemolysis growth on blood agar that causes no damage to the red blood cells