PCBI105L
June 2X, 202X
M3 - Exercise 2. Post-lab
Construct a matrix of the various Biochemical Tests by completing this table:
Biochemical Tests
Culture Media
pH Indicators
Positive Results
Carbohydrate
Fermentation Test
Carbohydrate
(sucrose, lactose or
maltose)
phenol red
yellow color
Indole Test
Tryptone broth
none
formation of a pink to
red color in the top
layer
Methyl-Red Test
Methyl Red- Voges
Proskauer (MRVP)
methyl red
red color
Voges-Proskauer Test
Methyl Red- Voges
Proskauer (MRVP)
methyl red
red color
Citrate Utilization Test
Simmon's Citrate agar
bromothymol blue
a color change from
green to intense blue
along the slant.
Catalase Test
nutrient agar
none
bubble formation
Oxidase Test
nutrient broth medium
none
dark purple
Coagulase Test
any fresh bacterial
cultures
none
clump or clots
formation
Urease Test
Christensen's urea
agar
phenol red
pink color
Hydrogen Sulfide Test
SIM (Sulfide Indole
Motility)
KIA (Kligler's Iron Agar)
TSI (Triple Sugar Iron)
none
black precipitate
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