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MICRO LAB MIDTERM PRACTICE TEST QUESTIONS WITH COMPLETE SOLUTION 2025, Exams of Microbiology

Newcastle UniversityMicrobiology

MICRO LAB MIDTERM PRACTICE TEST QUESTIONS WITH COMPLETE SOLUTION 2025

Typology: Exams

2024/2025

Available from 07/10/2025

DOCPASS
DOCPASS 🇬🇧

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MICRO LAB MIDTERM PRACTICE TEST QUESTIONS WITH COMPLETE
SOLUTION 2025
1. Where of you dispose of these items?
a.
Microscope slides
b.
gloves
c.
swabs
d. uncontaminated paper towels
e.
petri plates
f.
glassware, test tubes, flasks, bottles
2. Mordant for endospore staining technique:
Iodine,
Heat, Alcohol, acid-alcohol, malachite
green
3.
Microorganism that are dangerous and exotic
with
high risk of aerosol transmitted infections.
Rarely are
there treatment or vaccines for these
microorgan-
isms, and the Diseases they cause are
frequently fatal.
These microorganisms must be
housed and studied in lab with a minimum
rating.
Examples:Ebola virus, Marburg virus
4.
Indigenous microorganisms that can lead to dis-
eases of varying severity in healthy adults.
Examples: Staphylococcus aureus, streptococcus
pyogenes, Sal- monella
5.
Indigenous or exotic microorganisms that
cause se- rious or potentially lethal disease
through respirato-
ry transmission. Examples:
Mycobacteriu
m tuberculo-
sis, bacillus
anthraces,
francisella
tularensis
pf3
pf4
pf5
pf8
pf9
pfa
pfd
pfe
pff

Partial preview of the text

Download MICRO LAB MIDTERM PRACTICE TEST QUESTIONS WITH COMPLETE SOLUTION 2025 and more Exams Microbiology in PDF only on Docsity!

1 / 15

SOLUTION 2025

  1. Where of you dispose of these items? a. Microscope slides b. gloves c. swabs d. uncontaminated paper towels e. petri plates f. glassware, test tubes, flasks, bottles
  2. Mordant for endospore staining technique: Iodine, Heat, Alcohol, acid-alcohol, malachite green
  3. Microorganism that are dangerous and exotic with high risk of aerosol transmitted infections. Rarely are there treatment or vaccines for these microorgan- isms, and the Diseases they cause are frequently fatal. These microorganisms must be housed and studied in lab with a minimum rating. Examples:Ebola virus, Marburg virus
  4. Indigenous microorganisms that can lead to dis- eases of varying severity in healthy adults. Examples: Staphylococcus aureus, streptococcus pyogenes, Sal- monella
  5. Indigenous or exotic microorganisms that cause se- rious or potentially lethal disease through respirato- ry transmission. Examples: Mycobacteriu m tuberculo- sis, bacillus anthraces, francisella tularensis

2 / 15

SOLUTION 2025

a. sharps container b. biohazard can c. sharps container d. regular garbage can e. biohazard can f. discard cart Heat BSL 4

BSL 2

BSL 3

  1. Regulates the amount of light that reaches the slide Diaphragm
  2. Yellow

SOLUTION 2025

g themselves with microorganisms, ensures that coworkers and the work area do not become contaminated, and also en- all the above aseptic technique

SOLUTION 2025

sures that microbial cultures do not become unneces- sarily contaminated with unwanted microorganisms.

  1. Counter stain for gram staining technique safranin
  2. Immersion oil is used with which objective? 100X
  3. A medium causes bacteria to take on an appear- ditterential ance that distinguishes them from other bacteria
  4. You are using a microscope that has oculars with a magnification of 15X. There is 10X, 40X, 100X objective and you are currently using the 40X. What is the total magnification of the microscope as you are currently using it?

600X

  1. The distance between the bottom of the objective lens working distance and the slide is called
  2. Bacterial cell wall molecule that allows gram staining peptidoglycan
  3. The crystal violet complex in Gram staining is re- moved from gram negative bacteria by......
  4. You add nitrate A & B to a nitrate reduction tube. The tube turns red. This means that nitrate was reduced to. 95% ethanol nitrite
  5. Primary stain for endospore staining technique malachite green

SOLUTION 2025

  1. Produces metallic green colonies on EMB agar. Escherichia coli
  2. Which statement about gram staining is true? D a. the mordant in this staining technique is heat. b. gram negative bacteria retain the crystal violet stain as a result of their thick peptidoglycan layer c. gram positive bacteria will stain red d. cultures older than 18-24 hours may give erroneous results. e. All the above
  3. How many times should you flame your Loop when 5 performing a streak plate , if you only use one loopful of broth.
  4. species produce endospores Bacillus
  5. You need to observe the transparent internal (cy- toplasmic) structures of a bacterium. The stain we used in the lab only stain the cell membrane and en- dospores. Which microscope can be used to visualize the structures you need to observe?
  6. Determined by multiplying the power of the ocular lens times the power of the objective lens used is called what
    1. The ability of a microscope lens system to show two closely spaced objects as distinct and separate is called

SOLUTION 2025

  1. A sample taken by swabbing a door handle is a for mite sample? True or False Phase- Contrast microscope total magnification resolving power True

SOLUTION 2025

false optical density False E

SOLUTION 2025

  1. Most pathogenic bacteria are..... mesophiles
  2. List the reagents for the Gram stain technique in prop- crystal violet, gram's iodine, er order. ethanol, safranin
  3. After an endospore stain, the vegetative cells are what Green color?
  4. Mannitol salt agar is a media. a. selective b. differential c. blood containing d. A & B e. B & C
  5. Agar is a nutrient source for most bacteria. True or False
  6. EMB agar is a media. a. selective b. differential c. blood containing d. A & B e. B & C

D

False D

  1. This laboratory course will involve work with BSL___1& and BSL microorganisms only.
  2. Simplest form of microscopy where light is either passed

SOLUTION 2025

Converts the differences in optical density of cells into different shades of brightness. Allows for the visual- ization of morphology, external structures, and some internal structures. Stains are not required.

  1. When viewing a slide with the 10X objective, you focus course adjustment knob the microscope using the
  2. The diaphragm is opened completely when using the 100X objective
  3. Example of bacteria with a cocci morphology staphylococcus aureus
  4. example of bacteria with a rod shaped morphology pseudomonas aeruginosa
  5. is/are used to blot stained microscope slides dry bibulous paper
  6. The gram stain was originally designed, but failed, to differentiate bacteria from
  7. after an endospore stain, the endospores are what color? lung tissue green
  8. Complete lysis of red blood cells around a colony on a beta hemolysis sheep blood agar plate is known as.....
  9. Partial breakdown of the hemoglobin inside the red blood cells in a sheep blood agar plate produces a greenish

SOLUTION 2025

discoloration around colonies known as....

  1. complete lack of hemolysis on a sheep blood agar plate alpha hemolysis gamma hemolysis
  2. O/F glucose