Download micro 290 lab midterm Question and answers correctly solved 2025 and more Exams Microbiology in PDF only on Docsity!
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- what does PPE mean? personal protective equipment
- what does PPE include? includes but is not limited to lab coats, chemical aprons (if needed), and face masks
- what are the general most important safety habits when working in lab?
- what is type of micro- scope did you observe in the video?
- what is the magnifica- tion power of the com- pound microscope at 40X objective and 100X objective lens power?
- if a bacterium is 2 micrometers long, how many millimeters long does it appear under the 40X objective lens? how many millimeters long does it appear un- der the oil immersion lens? 7. staphylococcus aureus
2 / 16 do not touch face & mouth, disinfect your workplace (with 70% alcohol), wash hands frequently even when wearing gloves compound binocular light microscope the total magnification power of the compound microscope at 40X is 400X and the total magnification power of the compound microscope at 100X is 1000X the bacterium is 0.8mm long under 40X objective lens and 2mm long under the oil immersion lens (show work on this question)
- 2micro x 400 = 800 micro (0.8mm)
- 2 micro x 1000 = 2000 micro (2mm)
4 / 16 without contamination technique the process of transferring the microbes from one medium to another refers to the actual bacteria wire loop
- free of any types of germ; germ free
5 / 16 what does the word sterilizing mean?
- how are wire loops ster- ilized in the lab?
- what is the main pur- pose of isolation streak plate?
- what is the definition of bacterial colony?
- find and display picto- rial examples of isola- tion streak plates from internet that shows mix- ture of bacteria separat- ed into discrete colonies
- what is the meaning of bacterial smearing?
- what is the net electric charge on all biological membranes?
- what type of stains are mostly used for making bacterial staining tech- niques?
- what are 3 broad cate- gories of staining tech-
7 / 16 niques used in the labo- ratory?
- what is simple staining simple staining uses only one dye or stain and only allows you to observe and what is the main ad- the shape of bacteria and their arrangement vantage of it?
- what are the 3 most common shapes of bac- teria?
- staphylococcus aureus
- streptobacillus mono- liformis
- borrelia burgdorferi
- what is differential staining and what is the main advantage of it?
- give one example of a differential stain widely
8 / 16 b a c i l l u s ( r o d , o b l o n g ) coccus (sphere) spirillium (spiral, shape) type of stain that uses two or more stains/dyes. it allows for identification of specific groups of organisms gram stain most important and frequent type of stain used in the laboratory. used used in microbiology lab for ditterentiation for two major groups of bacteria based on their cell wall
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- gram-positive: purple or blue
- gram-negative: pink or red this is based on their distinct ditterences between gram-positive & negative bacteria turn ditterent colors based on their distinct ditterences between their cell walls gram-positive gram-negative
- gram-pos: staphylococcus aureus
- gram-neg: E. coli
- skin, oral cavity, and large intestine
11 / name 3 parts of your body that you would think have resident bac- teria as normal flora
- what does microbiome mean? genetic material of all the microbes including bacteria, fungi, protozoa, & viruses
- how do microbiomes af- - living inside the human body, each microbiome is 200 times the fect human health?
- review and understand key terms from case files! number of genes in the human genome.
- can weigh as much as 5lbs
- any alteration to bacteria of the gut has been linked to several diseases including: IBD, IBS, obesity, type II diabetes, and cancer :)
- what is gelatin and what a protein (polypeptide) commonly found in jello; molecular component is the molecular compo- is collagen nent of gelatin?
- what type of enzyme is released by some bacte- ria which digests (liqui- fies) gelatin? extracellular proteases called gelatinases
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- what type of enzyme is diastase is released and hydrolyzes by breaking the bonds between released by some bacte- glucose molecules ria which digest (hydrol- yses) starch?
- what type of reagent will iodine is useful in identification; interpreting results can be done by help in identification ofobserving if the iodine reacted with starch to form a dark brown color, if starch hydrolysis in agar not there is a clear zone around the bacterial growth cultures and how do you interpret the results?
- what is the meaning of catabolism?
- what is the purpose of Phenol Red broth and the breakdown of complex molecules in living organisms to form simpler ones, together with the release of energy, destructive metabolism commonly used to determine the test organism's ability to utilize a specific sugar as its source of energy production; it is a carbohydrate what is the components composed of pH indicator phenol red, the sugar of interest, salts, pep- of Phenol Red Carbohy- drate broth?
- list the 5 steps of DNA purification listed on pages 15 & 16 of your
14 / tone (soluble protein), Durham's tube neg ativ e fermentation: pink positive fermentation: yellow step 1: extraction step 2: lystase step 3: protease DNA extraction handout step 4: cold alcohol precipitation and explain each step
- gently chew the insides of your mouth and then rinse vigorously with water harvest the cells
16 / develops when a baby ingests C. botulinum spores, which are present in honey and soil; causes muscle weakness, which can lead to diflculty eating and breathing 70% alcohol
