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Laboratory Risk Assessment: Preparation of Inorganic Ash Spheres, Study notes of Laboratory Practices and Management

Laboratory Risk Assessment. Procedure. Preparation of Inorganic ash spheres. Level of Risk medium. Hazard. Risk. Recommended Control. Hydrogen peroxide -.

Typology: Study notes

2021/2022

Uploaded on 09/27/2022

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Laboratory Risk Assessment
Procedure Preparation of Inorganic ash spheres
Level of Risk medium
Hazard Risk Recommended Control
Hydrogen peroxide -
oxidising agent Personal injury -
chemical burns, eye injury, ?? Lab coat, safety glasses
and gloves must be
worn at all times.
?? Hydrogen peroxide
must be used in the
fume cupboard with the
window pulled down to
the safe working height
as indicated
Hydrogen peroxide -
oxidising agent explosion/fire ?? Spills on bench tops,
floors etc. should be
diluted with water
before mopping up.
Mopping up
concentrated hydrogen
peroxide with paper
towel can cause fires.
?? Samples with high
organic content should
be treated with cold
peroxide first and left to
stand for a short time
prior to heating
Unbalanced centrifuge Damage to centrifuge rotor
and risk of personal injury
- A broken rotor travelling
at high speed is capable of
breaking through the outer
casing of the centrifuge.
?? Ensure that the sample
buckets positioned
opposite each other on
the rotor weigh the
same.
The centrifuge does have
a light to indicate whether
it is properly balanced, but
this will only come on once
the speed of the rotor has
reached 1000 rpm and this
is often too late to prevent
damage.
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Laboratory Risk Assessment

Procedure (^) Preparation of Inorganic ash spheres

Level of Risk medium

Hazard Risk Recommended Control

Hydrogen peroxide - oxidising agent

Personal injury - chemical burns, eye injury,

?? Lab coat, safety glasses and gloves must be worn at all times. ?? Hydrogen peroxide must be used in the fume cupboard with the window pulled down to the safe working height as indicated Hydrogen peroxide - oxidising agent

explosion/fire (^) ?? Spills on bench tops, floors etc. should be diluted with water before mopping up. Mopping up concentrated hydrogen peroxide with paper towel can cause fires. ?? Samples with high organic content should be treated with cold peroxide first and left to stand for a short time prior to heating Unbalanced centrifuge Damage to centrifuge rotor and risk of personal injury

  • A broken rotor travelling at high speed is capable of breaking through the outer casing of the centrifuge.

?? Ensure that the sample buckets positioned opposite each other on the rotor weigh the same.

The centrifuge does have a light to indicate whether it is properly balanced, but this will only come on once the speed of the rotor has reached 1000 rpm and this is often too late to prevent damage.

Preparation of 0.3M Sodium Hydroxide Solution

Personal injury - chemical burns, eye injury

?? Lab coat, safety glasses and gloves must be worn at all times Preparation of 0.3M Sodium Hydroxide Solution - Heat generated by dissolution of NaOH

Personal injury - burns, flawed glassware may crack

?? Ensure glassware is free from cracks or flaws. ?? Cool the exterior of the flask in a bowl of cold water ?? Do not handle the base of the flask until it has cooled Preparation of 3M Hydrochloric Acid from Concentrated Acid

Personal injury - chemical burns, eye injury

?? Lab coat, safety glasses and gloves must be worn at all times ?? Conc. HCl must be used in the fume cupboard with the window pulled down to the safe working height as indicated ?? Always add acid to water. Use of 3M HCl Personal injury - chemical burns, eye injury, burns from hotplate.

?? Lab coat, safety glasses and gloves must be worn at all times ?? Allow beakers to cool before removing from the hotplate ?? Clear up any spillages immediately according to COSHH guidelines.