Validating Hydrogel Encapsulation of Cyanobacteria
When working with cyanobacteria, the strategies for attracting them into your hydrogel capsules
require adjustments because
cyanobacteria have unique physiological and environmental requirements compared to other
bacteria. Here's how the points can
be validated and tailored for cyanobacteria:
1. Chemotactic Attractants:
- Cyanobacteria do not rely on traditional chemotaxis. Instead, include nitrogen sources (e.g.,
nitrates or ammonium) or
phosphorus compounds in or near the hydrogel to attract cyanobacteria.
- Photosynthetically Active Radiation (PAR): Cyanobacteria are phototactic and will migrate toward
light.
Validation: Test the gradient by placing the hydrogel in a medium with varying nutrient
concentrations and monitor cyanobacteria
accumulation around the hydrogel.
2. Functionalizing the Hydrogel Surface:
- Modify the hydrogel with polysaccharides like agar, alginate, or cellulose to mimic natural
habitats.
- Add calcium ions to promote adhesion.
Validation: Use microscopy to observe if cyanobacteria preferentially attach to functionalized
hydrogels.
