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Genetics Pretest: Drosophila Genetics and Phenotype Ratios, Quizzes of Genetics

A pretest on genetics, focusing on chapters 4 and 5 related to drosophila melanogaster genetics. It provides information on why drosophila is used in genetic research, its life cycle, differences between male and female flies, the sex comb, how temperature affects the life cycle, and the results of specific crosses. It also discusses mendel's law pseudo-deviations, types of phenotypic ratio deviations, tools and materials needed for the practicum, and examples of ratio comparisons. The document aims to prepare students for a genetics exam by covering key concepts and practical aspects of drosophila genetics.

Typology: Quizzes

2019/2020

Available from 08/05/2024

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Pretest: Genetics
Chapter 4 (Monohybrid Crosses and Sex Chromosome Linked Genes in Drosophilla sp.) and
Chapter 5 (Imitation of Appearance and Modification of Phenotype Ratios)
1. Why is Drosophila melanogaster used in genetic research?
2. Name and explain the life cycle of Drosophila!
3. Write the differences between male and female Drosophila !
4. Explain what a sex comb is!
5. How does temperature affect the life cycle of Drosophila ?
6. What are the F1 and F2 results of crossing wild-type female Drosophilla and white-eyed
male Drosophilla ?
7. How do young and adult flies change?
8. How to determine sex in Drosophila ?
9. What is meant by pseudo-deviation of Mendel's laws?
10. Mention the types of phenotypic ratio deviations!
11. Mention events with different cross results using Mendelian comparisons!
12. Which method is suitable for calculating Mendel's ratio ratio? Explain!
13. In chapter 4, what tools and materials are needed in the practicum?
14. What tools and materials are needed for practicum chapter 5?
15. Mention examples of ratio comparisons of 16 types of genotype combinations!
Answer:
1. Drosophila melanogaster is often used because it is easy to obtain, maintain and breed,
its life cycle is short (around 2 weeks), it has 8 chromosomes so it is easy to observe.
2. Drosophila life cycle starts from zygote, embryo, egg, larva and develops into an imago
or adult fly. Spermatozoa fertilize eggs through small holes called microphylls. Fertilized
egg cells can be released immediately by the mother fly or remain in the uterus during the
early embryonic stages. The larval stage of the embryo is divided into 3 stages, namely
instars 1, 2, and 3. In instar 3, the larvae reach approximately 4.5 mm in length. At this
stage the larvae are very active and eat a lot. After that the larvae turn into pupae and
move to a dry place. After undergoing changes, fruit flies emerge from pupae and
become adult flies.
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Pretest: Genetics Chapter 4 (Monohybrid Crosses and Sex Chromosome Linked Genes in Drosophilla sp.) and Chapter 5 (Imitation of Appearance and Modification of Phenotype Ratios)

  1. Why is Drosophila melanogaster used in genetic research?
  2. Name and explain the life cycle of Drosophila!
  3. Write the differences between male and female Drosophila!
  4. Explain what a sex comb is!
  5. How does temperature affect the life cycle of Drosophila?
  6. What are the F1 and F2 results of crossing wild-type female Drosophilla and white-eyed male Drosophilla?
  7. How do young and adult flies change?
  8. How to determine sex in Drosophila?
  9. What is meant by pseudo-deviation of Mendel's laws?
  10. Mention the types of phenotypic ratio deviations!
  11. Mention events with different cross results using Mendelian comparisons!
  12. Which method is suitable for calculating Mendel's ratio ratio? Explain!
  13. In chapter 4, what tools and materials are needed in the practicum?
  14. What tools and materials are needed for practicum chapter 5?
  15. Mention examples of ratio comparisons of 16 types of genotype combinations! Answer:
  16. Drosophila melanogaster is often used because it is easy to obtain, maintain and breed, its life cycle is short (around 2 weeks), it has 8 chromosomes so it is easy to observe.
  17. Drosophila life cycle starts from zygote, embryo, egg, larva and develops into an imago or adult fly. Spermatozoa fertilize eggs through small holes called microphylls. Fertilized egg cells can be released immediately by the mother fly or remain in the uterus during the early embryonic stages. The larval stage of the embryo is divided into 3 stages, namely instars 1, 2, and 3. In instar 3, the larvae reach approximately 4.5 mm in length. At this stage the larvae are very active and eat a lot. After that the larvae turn into pupae and move to a dry place. After undergoing changes, fruit flies emerge from pupae and become adult flies.
  1. Difference:  Male Drosophila: tip of abdomen rounded, number of abdominal segments 5, genital comb in the form of 10 stiff black hairs on the distal surface of the last tarsus of the front legs  Female Drosophila: tip of abdomen elongated and tapered, number of abdominal segments 7, no genital comb.
  2. Sex comb is a characteristic of male flies. Sex combs can be used to identify the sex of a fruit fly in the first 2 hours after the fly hatches, when the fly's shape and pigmentation have not yet fully developed.
  3. Drosophila's life cycle is strongly influenced by temperature. If the larval culture is placed at a temperature of 10 ℃, it will prolong the larval stage. Meanwhile, if placed at a temperature of 30 ℃, it will cause the flies to become sterile or die. If the fly's entire life cycle is kept at a temperature of 10 ℃, it will cause the fly to live for 15 days. Meanwhile, if the temperature is 30 ℃, flies can survive for 10 days.
  4. The results of the F1 cross produce all flies that are wild type (red-eyed). Meanwhile, in the F2 results from the cross, it was found that ¾ had red eyes and ¼ had white eyes (mutant). All white-eyed flies in F2 were males.
  5. At first the flies are very long with undeveloped wings and light colored. When the fly is an adult, the wings will grow until the fly is round and dark in color.
  6. Drosophila has sex chromosomes of the XY type. Female fruit flies will have sex chromosomes consisting of a pair of X chromosomes. Meanwhile, male fruit flies will have sex chromosomes consisting of one X chromosome and one Y chromosome.
  7. Mendel's law pseudo-deviation is a form of crossing that produces different phenotypic ratios from the dihybrid basis according to Mendel's law. Mendel's laws of pseudo- deviation include gene interactions, cryptomery, epistasis-hypostasis, polymery, and complementarity.
  8. Phenotypic ratio deviation:  Polymery : there are many genes that are not alleles but affect the same character/trait. Example: crossing red and white wheat, the ratio of red : white is 15 : 1  Cryptomery : an event where a factor does not show its influence when it stands alone but its influence only becomes visible if there are other factors accompanying it. Example: crossing red and white Linaria maoccana , the ratio of red: white is 15: 1