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Exploring the Fundamental Building Blocks of Life: A Comprehensive Guide to Cell Biology -, Lecture notes of Biochemistry

This comprehensive document delves into the fascinating world of cell biology, providing a detailed exploration of the structural and functional components that make up the basic units of all living organisms. From the intricate details of prokaryotic and eukaryotic cells to the specialized organelles that drive cellular processes, this resource offers a comprehensive understanding of the building blocks of life. Covering topics such as the cell membrane, cytoplasm, nucleus, and various organelles, the document equips readers with a deep knowledge of the complex and dynamic nature of cells. Whether you're a student, researcher, or simply curious about the foundations of biology, this document serves as an invaluable reference, guiding you through the fascinating intricacies of cellular structure and function.

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2023/2024

Available from 08/26/2024

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PCCH104 - BIOCHEMISTRY
2nd Year 1st SEM | LABORATORY
Cell
โ—Cells are the structural and functional units of all
living organisms.
โ—Also called the โ€œbuilding blocks of life.โ€
โ—All cells are made from the same major classes of
organic molecules: nucleic acids, proteins,
carbohydrates, and lipids.
History of the Cell
โ—In 1665, Robert Hooke made an improved microscope
and discovered cells in cork.
Types of Cell
Prokaryotic Cell
โ—Unicellular organisms that do not develop or
di๎ž€erentiate into multicellular forms.
โ—Identical and capable of independent existence.
โ—No continuity or communication between the cells.
โ—Capable of inhabiting almost every place on the
earth.
โ—Include all bacteria and archaea (archaebacteria).
โ—Lack a nucleus and membranous organelles.
โ—Most of the functions of organelles , such as
mitochondria, chloroplasts, and the Golgi apparatus,
are taken over by the prokaryotic plasma membrane.
Architectural Regions of a Prokaryotic Cell
1. Appendages include ๏ฌ‚agella and pili (proteins
attached to the cell surface).
2. Cell envelope consisting of a capsule, a cell wall, and
a plasma membrane
3. Cytoplasmic region - contains the cell genome
(DNA) and ribosomes and various sorts of inclusions.
Eukaryotic Cell
โ—Include fungi, animals, and plants as well as some
unicellular organisms.
โ—About 10x the size of a prokaryote and can be as
much as 1000x greater in volume.
โ—Contain membrane-bound compartments in which
speci๏ฌc metabolic activities take place.
โ—Contains a nucleus.
โ—Has other specialized structures, called organelles
Major Parts of the Cell
1. Cell membrane
2. Cytoplasm
3. Nucleus
Cell Membrane
โ—Also called plasma membrane or plasmalemma
โ—Semi-permeable membrane
โ—All membranes are phospholipid bilayers with
embedded proteins.
Functions of the Cell Membrane
1. Delimits the cell from its surroundings.
2. Controls what gets in and out of the cell.
3. Provides attachment for the skeleton of the cell.
4. Receives and sends out stimuli.
5. Provides binding sites and receptors for enzymes and
other substances.
6. Allows cell-to-cell recognition.
7. Forms specialized junctions with cell membrane of
adjacent cells
Cytoplasm
โ—Jelly-like matrix that surrounds the nucleus and is
bound by the cell membrane.
โ—Contains dissolved nutrients, helps break down
waste products, and moves material around the cell.
โ—The living substance of the cell
๐’ธ๐“‰๐“๐“ƒ
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2nd Year 1st SEM | LABORATORY

Cell โ— Cells are the structural and functional units of all living organisms. โ— Also called the โ€œ building blocks of life .โ€ โ— All cells are made from the same major classes of organic molecules: nucleic acids, proteins, carbohydrates, and lipids. History of the Cell โ— In 1665, Robert Hooke made an improved microscope and discovered cells in cork. Types of Cell Prokaryotic Cell โ— Unicellular organisms that do not develop or dierentiate into multicellular forms. โ— Identical and capable of independent existence. โ— No continuity or communication between the cells. โ— Capable of inhabiting almost every place on the earth. โ— Include all bacteria and archaea ( archaebacteria ). โ— Lack a nucleus and membranous organelles. โ— Most of the functions of organelles , such as mitochondria, chloroplasts, and the Golgi apparatus, are taken over by the prokaryotic plasma membrane. Architectural Regions of a Prokaryotic Cell

  1. Appendages include flagella and pili (proteins attached to the cell surface).
  2. Cell envelope consisting of a capsule, a cell wall, and a plasma membrane
  3. Cytoplasmic region - contains the cell genome (DNA) and ribosomes and various sorts of inclusions. Eukaryotic Cell โ— Include fungi, animals, and plants as well as some unicellular organisms. โ— About 10x the size of a prokaryote and can be as much as 1000x greater in volume. โ— Contain membrane-bound compartments in which specific metabolic activities take place. โ— Contains a nucleus. โ— Has other specialized structures, called organelles Major Parts of the Cell 1. Cell membrane 2. Cytoplasm 3. Nucleus Cell Membrane โ— Also called plasma membrane or plasmalemma โ— Semi-permeable membrane โ— All membranes are phospholipid bilayers with embedded proteins. Functions of the Cell Membrane 1. Delimits the cell from its surroundings. 2. Controls what gets in and out of the cell. 3. Provides attachment for the skeleton of the cell. 4. Receives and sends out stimuli. 5. Provides binding sites and receptors for enzymes and other substances. 6. Allows cell-to-cell recognition. 7. Forms specialized junctions with cell membrane of adjacent cells Cytoplasm โ— Jelly-like matrix that surrounds the nucleus and is bound by the cell membrane. โ— Contains dissolved nutrients, helps break down waste products, and moves material around the cell. โ— The living substance of the cell

2nd Year 1st SEM | LABORATORY

Cytosol โ— The fluid portion of a cell's cytoplasm. โ— It contains water, free proteins, and other substances. โ— It is where a major part of cellular metabolism takes place. โ— It also contains the cytoskeleton

Organelle

Endoplasmic Reticulum โ— The principal protein-folding organelle for secretory and membrane proteins. โ— Proteins are folded, assembled, and post-translationally modified in the ER. โ— Concerned with intracellular transport โ— It is the largest membrane compartment within eukaryotic cells โ— Consisting of membranous tubules or flattened sacs (cisternae) that enclose a continuous lumen or space and extend throughout the cytoplasm Smooth Endoplasmic Reticulum Agranular โ€“ no attached ribosomes

  • It synthesizes lipids, phospholipids, and steroids like cholesterol.
  • Involved in the transport of fatty acids and other lipids โ— It is connected to the nuclear envelope. โ— The smooth ER is a continuous extension of the rough ER, located more distally from the nucleus. โ— It is usually more tubular in structure, forming a lacelike reticulum. Rough Endoplasmic Reticulum Granular โ€“ with ribosomes
  • Composed of flatten sealed sacs, which are contiguous with the nuclear membrane.
  • It is connected to the nuclear envelope.
  • It is more extensive than the smooth ER. โ— Rough ER is found throughout the cell but the density is higher near the nucleus and the Golgi apparatus. Mitochondria
  • Powerhouse of the cell
  • The major source of ATP synthesis in cells during aerobic respiration.
  • The mitochondrial inner membrane is highly invaginated, forming folded structures called cristae that protrude into the matrix of the mitochondrion. โ— ATP generation happens in the matrix Why are mitochondria similar to a bacteria? โ— They are organelles with a double membrane, approximately the size of a bacterium. โ— They have a circular DNA molecule and ribosomes with strikingly prokaryotic features. โ— They are motile. โ— They can undergo self replication in a manner similar to bacterial cell division Golgi Complex
  • Also called Golgi apparatus or Golgi body
  • Consists of disk-like membranes (cisternae) organized into stacks, or dictyosomes.
  • Stacked, flattened membranes
  • Its inner face (cis or entry face) near the rER

2nd Year 1st SEM | LABORATORY

Nucleolus โ— Membrane-less structure whose primary function is ribosomal RNA (rRNA) synthesis and ribosome biogenesis. โ— Generates ribosomes that are referred to as protein factories โ— Most prominent structure in the nucleus