Docsity
Docsity

Prepare for your exams
Prepare for your exams

Study with the several resources on Docsity


Earn points to download
Earn points to download

Earn points by helping other students or get them with a premium plan


Guidelines and tips
Guidelines and tips

COMMON LABORATORY TECHNIQUES AND APPARATUSES, Study notes of Chemistry

The Common Laboratory Techniques describe procedures for safety conducting an experiment.

Typology: Study notes

2022/2023

Uploaded on 09/09/2023

krishia-mae-dela-cruz
krishia-mae-dela-cruz 🇵🇭

5 documents

1 / 10

Toggle sidebar

This page cannot be seen from the preview

Don't miss anything!

bg1
ENGCHEM 101 Page 1 OF 10
Activity No. 2
COMMON LABORATORY TECHNIQUES AND APPARATUSES
I. COMMON LABORATORY TECHNIQUES
The Common Laboratory Techniques describe procedures for safety conducting
an experiment. Be sure to read each technique carefully before the laboratory session
for completing a safe and successful experiment.
A. Handling Liquid Chemicals
1. Opening a Reagent Bottle.
Grasp the reagent bottle with one hand and using the other hand, hold the
glass stopper between fingers and lift (Fig.A.1a). Never lay the stopper from a
reagent bottle on the laboratory bench. Impurities may be picked up and thus
contaminate the solution when the stopper is returned to the reagent bottle. Flat
top glass stopper should be laid upside down on the laboratory bench.
Fig.A.1. Removing a glass stopper from a reagent bottle.
2. Transferring Liquids to Another Container.
To transfer a liquid from one container to another, hold a stirring rod
against the lip of the container holding the liquid and pour it down the rod which
should touch the inside wall of the receiving vessel (Fig.A.2). Try not to transfer
more reagent than is needed, and do not return unused chemicals to the reagent
bottle.
pf3
pf4
pf5
pf8
pf9
pfa

Partial preview of the text

Download COMMON LABORATORY TECHNIQUES AND APPARATUSES and more Study notes Chemistry in PDF only on Docsity!

Activity No. 2 COMMON LABORATORY TECHNIQUES AND APPARATUSES I. COMMON LABORATORY TECHNIQUES The Common Laboratory Techniques describe procedures for safety conducting an experiment. Be sure to read each technique carefully before the laboratory session for completing a safe and successful experiment. A. Handling Liquid Chemicals

  1. Opening a Reagent Bottle. Grasp the reagent bottle with one hand and using the other hand, hold the glass stopper between fingers and lift (Fig.A.1a). Never lay the stopper from a reagent bottle on the laboratory bench. Impurities may be picked up and thus contaminate the solution when the stopper is returned to the reagent bottle. Flat top glass stopper should be laid upside down on the laboratory bench. Fig.A.1. Removing a glass stopper from a reagent bottle.
  2. Transferring Liquids to Another Container. To transfer a liquid from one container to another, hold a stirring rod against the lip of the container holding the liquid and pour it down the rod which should touch the inside wall of the receiving vessel (Fig.A.2). Try not to transfer more reagent than is needed, and do not return unused chemicals to the reagent bottle.

Fig.A.2. Transferring a liquid from a reagent bottle.

  1. Reading the Volume of a Liquid For exacting the measurements of liquids in graduated cylinders, pipettes, burettes, and volumetric flask, the solution’s volume is read at the bottom of the meniscus. Read with the eye horizontal to the liquid’s surface (Fig.A.3). A clear or transparent liquid is read more easily by positioning the top edge of a black mark (made on a white card) just below the level portion of the liquid. The black background reflects off the bottom of the lower meniscus and better defines the liquid’s level. Substituting a finger for the black mark on the white card will work, but it is not as effective. Fig. A.3. Reading the volume of the liquid.
  2. Testing for the Odor and Taste of Chemicals Chemicals should not be tasted nor tested for odor unless specially directed to do so. Working with toxic vapors must be done in a ventilating hood. To test the odor of a substance, waft a bit of vapor towards the nose as shown in Fig A.4. Do not inhale the vapor directly from the test tube. Never hold your face directly over vapors!

flask tongs laboratory bench; allow to cool by setting the flask on a wire gauze. See Fig. A.5b. Fig. A.5b. Heating liquid in an Erlenmeyer Flask. c. Beaker (or flask). Support the beaker (or flask) on wire gauze. To avoid bumping (sudden formation of superheated liquid near the flame), place a glass stirring rod (or use boiling chops) in the beaker. Position the flame directly under the tip of the stirring rod (Fig.A.5c). Place a second supporting ring around the top of the beaker (or flask) to prevent it from being accidentally knocked off. d. In hot water Bath. This is used to heat a small quantity of solution in a test tube that needs to be held at a constant temperature over period of time (Fig. A.5d). if the solution is to be heated in a beaker or Erlenmeyer flask, use a larger as water bath filled to about ¼ - full, and heat to the desired temperature. Fig. A.5c.Heating liquid in a Fig. A.5d. Heating liquid in a Beaker. Hot water bath.

  1. Evaporation of liquids a. Nonflammable liquids may be evaporated in an evaporating dish with a gentle, direct flame (Fig. A.6.a) or over a steam bath (Fig. A.6.b), which is more efficient rather than rapid boiling. Avoid inhaling the vapors. b. Flammable liquids may be similarity evaporated substituting a heating mantle for the Bunsen flame. The use of fume hood or an improvised hood is suggested if large amounts are evaporated in a laboratory with inadequate ventilation; consult with your laboratory instructor. Fig.A.6.a. Evaporation of nonflammable Fig.A.6.b. Evaporation of non- liquid over a low, direct flame. flammable liquid over a steam bath. B. Handling Solid Chemicals Transferring a Solid Reagent First, read the label on the bottle twice to be sure of using the correct reagent. If the bottle has a glass stopper, place it with the topside down on the bench (Fig. B.1). If it has a screw cap, remove and set it on the bench, inner inside facing up. Hold the bottle with the label against your hand, tilt, and roll rock and forth. Try not to dispense more reagent than needed. Do not insert a spatula or other object into the bottle unless your O specifically tells you to. If too much reagent is taken, do not return excess to the bottle but rather share it with other students. Recap when finished. Fig. B.1. Transferring solid reagent.

Fig. C.2a. Centrifuge Fig. C.2b. Centrifugation Gravity Filtration. Gravity filtration has the advantage that it is simple and economical. It is most effective when the amount of solid is to be isolated is small. If a large amount of solid or a gelatinous solid is to be isolated, this method is not used because the filter paper may becomed clogged. In this case, vacuum filtration is most suitable.

  1. General Procedures for Filtration a. Allow the precipitate to settle to the bottom of the container. b.Wet the filter paper with a small amount of water or a liquid in which the precipitate is insoluble. c. Decant (pour off) the bulk of the solution into the funnel using a stirring rod. This step ensures the filtration will not be slowed by the precipitate clogging the filter paper prematurely since most of the solution will have already filtered through. d. Mix the precipitate with the remaining solution and dump the resulting precipitate slurry into the funnel. Simultaneously, spray a stream of solution from a wash bottle to rinse out any slurry adhering to the walls of the container. (See Fig C.1.) Remember: the liquid or solution used for washing the precipitate must be one in which the precipitate is insoluble. Fig. C.1. Transferring a precipitate
  • Wet a rubber policeman or glass rod with wash liquid and use it to help transfer any precipitate still clinging to the container walls.
  • Use was bottle to rinse out whatever precipitate remains.
  • Typically 5 - to 10 mL of a wash solution are drawn through the funnel to wash the precipitate. This may be repeated several times to ensure that only pure precipitate remains.
  • Allow air to be drawn through the filter paper several minutes to dry the precipitate partially. e. Usually precipitates are washed or rinsed to remove impurities, unless the filtrate is the desired component. f. Lift a corner of the filter paper with a spatula and then use the corner to pry up the rest of the kilter paper. Carefully transfer the filter paper and precipitate to a previously weighed watch glass. Allow the precipitate to air dry, or oven dry (if melting point is above 70⁰C).
  1. Folding a Filter Paper Fold the filter paper in half (Fig. C.2). then refold to within about 10⁰ of a 90⁰ fold, tear off the corner unequally, and open. The tear enables a close seal to be made across the paper’s folded portion when placed in a funnel. Fig. C.2 technique in folding a filter paper D. Inserting a Glass Tubing into a Stopper To insert glass tubing (including thermometers, long-stemmed funnels, thistle tubes, etc.) through a rubber stopper, first lubricate the tube and stopper with water or glycerol. Hold the tubing with a cloth near the end to be inserted, and push with a twisting motion. (If you twist a long-stemmed funnel or thistle tube by the large end, it is easily broken.)

COMMON LABORATORY APPARATUSES and ITS USES

  1. Balance – for measuring mass
  2. Beaker – to hold, mix, and heat liquids
  3. Bunsen burner – as heat source in the absence of flammable materials
  4. Buret – for dispensing accurate volume of a liquid
  5. Clay triangle – to support a crucible during heating
  6. Crucible – for holding chemicals during heating to very high temperatures
  7. Crucible tongs – to hold crucibles
  8. Erlenmeyer flask – to hold mix chemicals. The small neck is to facilitate mixing without spilling
  9. Evaporating dish – to heat liquids for evaporation
  10. Funnel – for Filtration; also to transfer liquids or fine-grained materials into containers with small openings.
  11. Graduated Cylinder – to measure a precise volume of a liquid
  12. Mortar and pestle – to crush and grind materials
  13. Ring clamp – used with a ring stand to hold glassware, such as beaker or funnel
  14. Ring stand – to support laboratory glassware, water bath, etc.
  15. Spatula – to transfer solids
  16. Stirring rod – for stirring and mixing
  17. Test tube – to hold and mix liquids
  18. Test tube holder – to hold test tube during heating
  19. Test tube rack – to hold several tubes at one time
  20. Thermometer – to measure temperature
  21. Utility/ iron clamp – to secure glassware to a ring stand
  22. Volumetric flask – to prepare solutions of accurate volume
  23. Volumetric pipet – to measure small amounts of liquid very accurately; draw in or suck the liquid using an aspirator (never use your mouth)
  24. Wash bottle – to rinse parts of glasswares and to add small quantities of water
  25. Watch glass – to hold solids while weighing or to cover a beaker
  26. Wire gauze – to support a container, such as beaker, on a ring stand during heating