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Chapter 8 : Microbial Metabolism | MICB - Microbiology, Quizzes of Microbiology

Class: MICB - Microbiology; Subject: Microbiology; University: Queens University; Term: Forever 1989;

Typology: Quizzes

2019/2020

Uploaded on 09/29/2020

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TERM 1
Metabolism
DEFINITION 1
Sum of all biochemical reactions in an organismincludes
chemical reactions that break down complex molecules
(catabolism) and those that build complex molecules
(anabolism).
TERM 2
2 types of reaction
DEFINITION 2
1. Catabolic2. Anabolic
TERM 3
Catabolic
DEFINITION 3
Breakdown large molecules - broken down into smaller
molecules
TERM 4
Anabolic
DEFINITION 4
small molecules - large moleculesEnderorganic
TERM 5
Endergonic
DEFINITION 5
Anabolic reactionRequire energy
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Metabolism

Sum of all biochemical reactions in an organismincludes chemical reactions that break down complex molecules ( catabolism ) and those that build complex molecules ( anabolism ). TERM 2

2 types of reaction

DEFINITION 2

  1. Catabolic2. Anabolic TERM 3

Catabolic

DEFINITION 3 Breakdown large molecules - broken down into smaller molecules TERM 4

Anabolic

DEFINITION 4 small molecules - large moleculesEnderorganic TERM 5

Endergonic

DEFINITION 5 Anabolic reactionRequire energy

Exergonic

Produce energy into ATP TERM 7

cells balance anabolic and catabolic reaction

DEFINITION 7 Large molecules - Catabolic reaction - Small molecules +ATP

  • Anabolic TERM 8

cells can be classified

DEFINITION 8 Energy and carbon TERM 9

classification by carbon and energy sources

DEFINITION 9

  1. C Source - autrophs and heterotrophs2. E Source - phototrophs and chemotrophs TERM 10

Autotrophs

DEFINITION 10 convert inorganic carbon dioxide into organic carbon;

Photoautotrophs

Energy source is light and carbon source inorganic All plants, algae, cyanobacteria, green and purple sulfur bacteria TERM 17

Photoheterotrophs

DEFINITION 17 Energy source is light and carbon source is organic Green and purple nonsulfur bacteria, heliobacteria TERM 18

Oxidation and reduction

DEFINITION 18 Energy often transferred between molecules through high- energy electronsCellular electron carriers accept high-energy electrons from foods and later serve as electron donors in subsequent redox reactions. FAD/FADH2, NAD+/NADH , and NADP+/NADPH are important electron carriers. TERM 19

Molecule oxidized

DEFINITION 19 Lost electrons part of hydrogen TERM 20

Molecule reduced

DEFINITION 20 Gained electrons part of hydrogen and redox reactions

Adenosine triphosphate (ATP)

Adenosine triphosphates an organic compound and hydrotrope that provides energy to drive many processes in living cells Adenosine triphosphate (ATP) serves as the energy currency of the cell, safely storing chemical energy in its two high-energy phosphate bonds for later use to drive processes requiring energy. TERM 22

ATP 3 parts

DEFINITION 22 Ribose sugar, adenine and phosphate group TERM 23

ATP

DEFINITION 23 Used as energy currency in the cells Used to couple exergonic (make ATP) to endergonic (require ATP) reactions TERM 24

Adenosine

DEFINITION 24 Adenne + Ribose TERM 25

ATP Average bacterial needs

DEFINITION 25 ~3x10^6 ATP/sec to supply energy needs

Ribozymes

Enzymes made of RNA TERM 32

Substrate

DEFINITION 32 Binds at a enzymes active sitea competitive inhibitor most structurally resembleThis process typically alters the structures of both the active site and the substrate, favoring transition-state formation; this is known as induced fit. TERM 33

induced fit model : enzyme-substrate

complex

DEFINITION 33

  1. Substrate enter active sites of enzyme2. Enzymes/ substrate complex form3. substrate is converted to products4. Products : Products leave the active site of the enzyme TERM 34

Non-protein

DEFINITION 34 Some enzymes require additional non-protein factors to function properly TERM 35

Cofactor

DEFINITION 35 Inorganic substance (e.g. ions- Mg2+,Zn2+)are inorganic ions that stabilize enzyme conformation and function

Coenzyme

small organic mole (e.g. NAD+FAD)organic molecules that help enzymes workcorrectlyare organic molecules required for proper enzyme function and are often derived from vitamins. TERM 37

Apoenzyme

DEFINITION 37 Enzyme lacks cofactor/coenzyme (inactive)An enzyme lacking a cofactor or coenzyme TERM 38

Holoenzyme

DEFINITION 38 Enzyme is bound to cofactor/coenzyme (active) TERM 39

process

DEFINITION 39 Cofactor or coenzyme > activation>substrate1. Aponenzyme becomes active by binding of coenyme or cofactor to enzyme2. Holoenzymes is formed when associated cofactor or coenzyme binds to the enzyme's active site TERM 40

how enzymes increase the rate of chemical

reactions

DEFINITION 40 Enzymes lower E(activation E ) needed to break /form chemical bonds required in chemical reactionSubstrate binds to active site in a way in which bonds are more likely to be broken/formed

effect of temperature on enzyme activity

Enzyme activity > Optimum temperature >Denature TERM 47

Denature Enzyme

DEFINITION 47 Enzyme is denature at low temperatureDenature enzyme is not going to function TERM 48

Kinetic energy

DEFINITION 48 At low kinetic energy there are few interaction between enzymes and substrateDecrease in temperature = decrease Energy TERM 49

effect of pH on Enzyme activity

DEFINITION 49 Enzyme Activity > optimum ph > PH TERM 50

High PH

DEFINITION 50 Denaturation

Effect of substrate concentration on enzyme

activity

Increase concentration - Plateau TERM 52

Maximum velocity

DEFINITION 52 all enzyme activity sites are full TERM 53

Competitive inhibition

DEFINITION 53 inhibitor binds at active site - substrate cant bindTo be effective [ inhibitor] needs to be ~ equal to [ substrate]regulate enzymes by binding to an enzymes active site, preventing substrate binding. TERM 54

Noncompetitive inhibition

DEFINITION 54 allosteric inhibition - inhibitor binds to allosteric site (allosteric) inhibitors bind to allosteric sites , inducing a conformational change in the enzyme that prevents it from functioning. TERM 55

allosteric site

DEFINITION 55 Binding of inhibitor to allosteric site causes active site to change shapeCan be less than substrate

Aerobic respiration

Requires oxygena cell requires oxygen as the final electron acceptor.A cell also needs a complete Krebs cycle, an appropriate cytochrome oxidase, and oxygen detoxification enzymes to prevent the harmful effects of oxygen radicals produced during aerobic respiration. TERM 62

Anaerobic respiration

DEFINITION 62 Doesnot require O2Use alternative electron transport system carriers for the ultimate transfer of electrons to the final non- oxygen electron acceptors. TERM 63

Fermentation

DEFINITION 63 incomplete breakdown of carbohydrate produces less energyMany bacteria cells in multicellular organisms Fermentation uses an organic molecule as a final electron acceptor to regenerate NAD+ from NADH so that glycolysis can continue. Fermentation does not involve an electron transport system, and no ATP is made by the fermentation process directly. Fermenters make very little ATPonly two ATP molecules per glucose molecule during glycolysis. TERM 64

reaction for cellular

respiration

DEFINITION 64 C6H1206 Glucose >6CO2+6H20+ ATP TERM 65

Glucose

DEFINITION 65 Breakdown of glucose is one key E-releasing chemical reactions in most organisms1 mole of glucose has 686, cal of energyAerobic cellular respiration harvest ~40% of this energymolecules is not produced during the breakdown of phospholipids

Glycolysis :EMP pathway

Breakdown of 1 glucose (6C) to 2 pyruvates (3C)Take place in the cytosol1 glucose > 2 pyruates Glycolysis is the first step in the breakdown of glucose, resulting in the formation of ATP, which is produced by substrate-level phosphorylation ; NADH; and two pyruvate molecules.Glycolysis does not use oxygen and is not oxygen dependent. TERM 67

Different types of glycolysis

DEFINITION 67

  1. Enterner-Doudoroff pathway2. Pentose phosphate pathway3. Embden-Meyerhoff-Parnas (EMP) pathway TERM 68

Embden-Meyerhoff-Parnas (EMP) pathway

DEFINITION 68 Most commonly, glycolysis occurs by the EMP pathway. TERM 69

Endergonic step - E-investment phase

DEFINITION 69 uses up to 2 ATPs +4ATP - 2 ATP = +2 ATP TERM 70

Substrate-level phosphorylation

DEFINITION 70 A P group is transferred from a hi-E metabolite to ADP, producing ATP

EMP pathway : Energy

payoff

Oxidized H-C-O-P-O>6. Removed H(e) > reduced NAD++Pi NADH+H+7. 1,3-bisphospho-glycerate - ADP ATP - Substrate level phosphorylation 8.- 3-phosphoglycerate >9.2- phosphoglycerate > 10.Phosphoenolpyruvate (PEP) TERM 77

transition reaction

DEFINITION 77 x2 for every glucose that cellular respirationDoes not produce any ATP directlyNADH are produced1. Pyruvate - A carboxyl group is removed from pyruvate releasing carbon dioxide2. NAD+ is reduced to NADH3. An acetyl group is transferred to coenzyme A, resulting in acetyl CoAATP not made by substrate-level phosphorylation TERM 78

prokaryotes

DEFINITION 78 takes place along cell membraneCytoplasmThe cytoplasmic membraneis the location of electron transports systems in prokaryotesIn prokaryotes, hydrogen ions pumped by the electron transport system of photosynthetic membranesto the outside of the plasma membrane TERM 79

Eukaryotes

DEFINITION 79 Happens inside mitochondria matrixMitchochondrial matrix TERM 80

Pyruvate

DEFINITION 80 oxidized to acetyl-CoACo2 is released (1 pyruvate >2 acetyl- CoA) - making bread riseproducts is made during Embden- Meyerhof glycolysismolecule typically serves as the final electron acceptor during fermentationnot a commercially important fermentation product?

Mitochondria Matrix

Start pyruvate end acetyl -CoANo ATP provided directly TERM 82

Kreb's Cycle

DEFINITION 82 2X for every glucose that entered cellular respirationAlso called the citric acid cycleProkaryotes- cytoplasmEukaryotes- mitochondrial matrixProduces ATP and NADPH + FAD H2Glucose completely broken down into Co TERM 83

Electron transport system

DEFINITION 83 Some of the protein complexes contain H+ channels - Energy obtained from e-transfer is used to pump H+ to one side of the membraneis composed of a series of membrane-associated protein complexes and associated mobile accessory electron carriers.The ETS is embedded in the cytoplasmic membrane of prokaryotes and the inner mitochondrial membrane of eukaryotes. TERM 84

Electrochemical gradient

DEFINITION 84 E is now stored asElectrochemical gradient of H+ ions - Proton motive force (PMF) TERM 85

ATP synthase complex

DEFINITION 85 Made of 24 polypeptides

Oxidation

The loss of an electron from a molecule is