MARY ROSELLE DESIREE A. VERALLO MR. JESUS GIOVANNI A. DILANCO
3BSMT1
MT 113 – HISTOPATHOLOGIC AND CYTOLOGIC TECHNIQUES, LEC.
ASSIGNMENT #1
True or False. On the blanks, write True if the statement is correct or False if it is incorrect.
1. Tissue degradation starts as soon as its blood supply is interrupted.
2. Warm ischemia is beyond the control of the histopathology laboratory.
3. Prevention of cold ischemia requires immediate transport of tissue from the
operating room (OR) to the laboratory.
4. The proper ratio of fixative to tissue is 5:1.
5. Poorly fixed tissue will not process, cut, or stain well.
6. ASCO-CAP guidelines recommend a fixation time of a minimum of 6 hours and a
maximum of 48 hours.
7. A properly filled-out histopathology request form includes patient demographics,
history, pertinent physical and laboratory as well as imaging findings to help
pathologists arrive at the correct tissue diagnosis.
8. Unlabeled specimens are acceptable for accessioning.
9. Barcoding specimens greatly help in specimen identification along with patient
demographics.
10. False-negative results may happen when the tissues used for targeting
therapy testing are not properly fixed.
Enumeration. Give 4 pre-analytical factors that impact tissue diagnosis, prognostication,
and theranostics.
Properly handling the tissue specimens not only during and after their delivery to the
laboratory is important, especially in histopathology. If the not properly handled, there are
several factors that could affect the integrity of the specimens which could also affect the
tissue processing. Pre-analytical factors are factors that could affect the tissue because it
already produced artifacts making the tissues suboptimal for tissue preparation even before
arriving at the laboratory. The following are the pre-analytical factors:
1. Warm Ischemia
2. Cold Ischemia
3. Fixation
4. Accessioning Procedure
True
True
True
False
True
True
False
False
True
True
