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Analysis of Urine and Bodily Fluids: Midterm Assignment #1 - Urine Testing, Assignments of Bacteriology

Information on various urine tests used in laboratories for diagnosing different health conditions. The tests include ph reagent strip, urine specific gravity reagent strip, and protein reagent strip. Each test is described in detail, including the principle behind it, positive and negative interferences, and clinical correlations. The document also mentions correlations with other tests such as nitrite, leukocytes, and microscopic examination.

Typology: Assignments

2022/2023

Uploaded on 01/23/2024

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MARY ROSELLE DESIREE A. VERALLO MS. STEPHANY MAE CHI
3BSMT1 MT 111.1 – ANALYSIS OF URINE AND BODILY FLUIDS, LAB.
MIDTERM ASSIGNMENT #1
TESTS PRINCIPLE OF THE
TEST
POSITIVE/NEGATIVE
COLOR REACTIONS
POSITIVE
INTERFERENCES
NEGATIVE
INTERFERENCES CLINICAL CORRELATIONS
pH Reagent Strip Double-pH indicator
system. It could give
distinct color changes.
- Uses methyl red
and bromothymol
blue as an
indicator.
- Methyl red changes
from red to yellow
with a pH of 4 to 6.
- Bromothymol blue
turns yellow to deep
blue if the pH is 6 to
9.
- Runover from the adjacent pads
- Growth of the bacteria in the urine
- Specimens are already old
- No interference known
- Identification of respiratory
or metabolic acidosis,
ketosis, or alkalosis
- Identification of the defects
in renal tubular secretion
and reabsorption of acids
and bases which could be
an indicator of renal tubular
acidosis
- Early identification of renal
calculi formation and
prevention
- Early treatment for UTIs
- Identification of crystals that
could harm the urinary
system
- Determination of
unsatisfactory specimens
Correlation with other tests:
- Nitrite
- Leukocytes
- Microscopic such as
reporting of casts
Urine Specific Gravity Reagent
Strip
Ionic solutes present in
the urine cause protons
to be released from a
polyelectrolyte. If the
protons are released,
- Uses bromothymol
blue as an
indicator.
- Alkaline (negative)
would be blue with
- High
concentrations
of protein
which is
approximately
- Highly alkaline
urine (greater
than 6.5)
- In chem strips,
the glucose
- Monitors the hydration and
hydration
- Loss of renal tubular
concentrating ability
- An indication of Diabetes
pf3
pf4
pf5

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MARY ROSELLE DESIREE A. VERALLO MS. STEPHANY MAE CHI

3BSMT1 MT 111.1 – ANALYSIS OF URINE AND BODILY FLUIDS, LAB.

MIDTERM ASSIGNMENT

TESTS

PRINCIPLE OF THE

TEST

POSITIVE/NEGATIVE

COLOR REACTIONS

POSITIVE

INTERFERENCES

NEGATIVE

INTERFERENCES

CLINICAL CORRELATIONS

pH Reagent Strip Double-pH indicator system. It could give distinct color changes.

  • Uses methyl red and bromothymol blue as an indicator.
  • Methyl red changes from red to yellow with a pH of 4 to 6.
  • Bromothymol blue turns yellow to deep blue if the pH is 6 to
      • Runover from the adjacent pads
      • Growth of the bacteria in the urine
      • Specimens are already old
      • No interference known
        • Identification of respiratory or metabolic acidosis, ketosis, or alkalosis
        • Identification of the defects in renal tubular secretion and reabsorption of acids and bases which could be an indicator of renal tubular acidosis
        • Early identification of renal calculi formation and prevention
        • Early treatment for UTIs
        • Identification of crystals that could harm the urinary system
        • Determination of unsatisfactory specimens Correlation with other tests:
        • Nitrite
        • Leukocytes
        • Microscopic such as reporting of casts Urine Specific Gravity Reagent Strip Ionic solutes present in the urine cause protons to be released from a polyelectrolyte. If the protons are released,
  • Uses bromothymol blue as an indicator.
  • Alkaline (negative) would be blue with
  • High concentrations of protein which is approximately
  • Highly alkaline urine (greater than 6.5)
  • In chem strips, the glucose
  • Monitors the hydration and hydration
  • Loss of renal tubular concentrating ability
  • An indication of Diabetes

the pH decreases producing a color change on the indicator. a specific gravity of

  • Acidic (positive) could give a varied shade of green to yellow having a specific gravity of
    1. equal to 100 – 500 mg/dL
  • Ketoacidosis and urea might be greater than 1 g/dL insipidus
  • Determination of unsatisfactory specimens due to low concentration Protein Reagent Strip Protein error of indicators. If the pH is held constant by a buffer, the indicator releases hydrogen ions due to the proteins that could be in the urine.
  • Uses tetrabromophenol blue and acid buffer as an indicator.
  • The negative color would be yellow indicating the absence of proteins as it is also the normal color of the indicator.
  • Positive results of protein would give a color of various shades of green to blue. This demonstrates that the protein concentration is increasing.
  • Highly buffered interference alkaline urine
  • Pigmented specimens or phenazopyridine
  • Quaternary ammonium compounds (detergents)
  • Presence of antiseptics such as chlorhexidine
  • Loss of buffer from prolonged exposure if the strip to the specimen reagent
  • Has a high specific gravity
  • There are other proteins than albumin
  • Patient has microalbuminuria
  • Pigmented specimens or phenazopyridine
  • Prerenal  Indicator of intravascular hemolysis, muscle injury, or multiple myeloma  There could be an acute phase of reactants
  • Tubular Disorders  Indication of Fanconi syndrome or severe viral infections  Too much exposure to toxic agents or heavy metals
  • Renal  An indicator for glomerular disorders, immune complex, amyloidosis, diabetic nephropathy, preeclampsia, or orthostatic or postural proteinuria  Exposure to toxic agents  The patient might do a

with antibody-coated blue latex particles. The intensity comparison of two blue bands determines the albumin concentration of the patient. particles as a reagent.

  • Has two bands where the bound particles or albumin stays in the top band while unbound particles are in the bottom band.
  • Negative result shows darker bottom band indicating less than 1.2 mg/dL of albumin concentration.
  • The darker color of the top shows a positive result as it detects 2.0 to 8. mg/dL of albumin in the patient.
  • For normal results, it shows equal bands and has 1.2 to 1. mg/dL of albumin concentration. Albumin: Creatinine Ratio Clinitest Microalbumin Strips/Multistix -Pro Sensitive albumin tests related to creatinine concentration to correct for patient hydration.
  • Uses a dye DIDNTB for albumin and CuSO4. 3,3’,5,5’- TMB, and DBDH for creatinine.
  • For albumin, its negative color would be pale green and aqua blue for positive results.
  • Bloody urine
  • Highly colored mask results due to taking of certain drugs and ingestion of beets
  • Negative color is orange and positive color is blue for the albumin reaction. Glucose Reagent Strip - - - - Ketone Reagent Strip - - - - Blood Reagent Strip - - - - Bilirubin Reagent Strip - - - - Urobilinogen Reagent Strip - - - - Nitrite Reagent Strip - - - - Leukocytes Estrase Reagent Strip