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AAB MT Molecular Diagnostics Enzymes Exam Questions And Answers
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EcoR1, HindIII, BamHI, NotI, and PacI are all examples of which class of restriction enzymes? - correct answer ✅Class II This class of restriction enzymes account for at least 90% of enzymes used in molecular biology? - correct answer ✅Class IIP (Palindromic specificity) How many base pairs are normally recognized by Class II restriction enzymes? - correct answer ✅4-8 base pairs (6-8 allows for larger fragments) This restriction enzyme class typically recognizes modified (methylated) DNA? - correct answer ✅Class IV This enzyme degrades ssDNA from either the 5' or 3' end? - correct answer ✅Exonuclease VII
This enzyme is one of the few enzymes that has 5' exonuclease activity? - correct answer ✅Exonuclease VII What is the enzyme that cleaves the extra nucleotides from the 5' end of a tRNA precursor. Most forms of bacterial RNase P have a catalytic RNA subunit? - correct answer ✅RNase P (Ribonuclease P) What is the enzyme that synthesizes DNA by linking together deoxynucleoside monophosphates (dNMPs) in the order dictated by the complementary sequence of nucleotides in a template DNA strand? - correct answer ✅DNA polymerase What is the enzyme that cleaves other proteins? - correct answer ✅Protease What is the enzyme that degrades RNA? - correct answer ✅RNase (Ribonuclease)
What enzyme facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond? - correct answer ✅DNA Ligase What enzyme performs base excision repairs (BER) that is required for DNA maintenance, replication, recombination, and drug resistance? - correct answer ✅DNA polymerase beta What enzyme is involved in initiating DNA replication and lacks 3' exonuclease activity for proofreading errors? - correct answer ✅DNA Polymerase Alpha What enzyme is used in DNA synthesis (leading and lagging strand) and DNA repair? - correct answer ✅DNA Polymerase Delta What enzyme plays a major role in leading strand DNA synthesis and nucleotide/base excision repair? - correct answer ✅DNA Polymerase Epsilon
What enzyme participates as a backup in base excision repairs and re-synthesizing missing nucleotides during non-homologous end joining? - correct answer ✅DNA Polymerase Lambda What enzyme is replicates the ends of a chromosome because normal DNA polymerase is unable to do so? - correct answer ✅Telomerase What enzyme has exonuclease activity and is the only DNA polymease found in mitochondria? - correct answer ✅DNA Polymerase Gamma