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AAB MT Exam Hem Lab Methods Questions And Answers
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What type of specimen is used for hematology testing? - correct answer ✅EDTA What type of specimen is used for coagulation testing? - correct answer ✅3.2% citrated plasma When should a 3.2% citrated plasma be rejected? - correct answer ✅If the specimen is clotted, hemolyzed or QNS. How is platelet poor plasma obtained from a 3.2 citrated plasma specimen? - correct answer ✅By centrifuging the specimen. In a coagulation specimen, what is the ratio between anticoagulant and whole blood? - correct answer ✅1:9 ratio In a coagulation specimen, what is the ratio between whole blood and anticoagulant? - correct answer ✅9:1 ratio
What is the formula for the Red Blood Cell (RBC) Count? - correct answer ✅(Cells counted X Dilution X 10) / Number of square millimeters counted What is a common source of errors for the RBC Count? - correct answer ✅Cold agglutinins (due to cold antibodies present). Why is platelet poor plasma essential for coagulation testing? - correct answer ✅Platelets initiates the coagulation cascade (results in shorten result times). Cold auto-antibodies cases what errors in the CBC test? - correct answer ✅A falsely decreased HCT and RBC, plus a falsely elevated MCV and MCHC (greater than 37). If a patient has a cold auto-antibody, what can be done to obtain a correct CBC? - correct answer ✅Incubate specimen at 37 C, reanalyze specimen immediately after incubation, and immediately make the peripheral smear.
When using the Cyanmethemoglobin method for determining hemoglobin concentration, how can lipemic samples be corrected?
correct answer ✅Using saline replacement technique (one volume of saline per one volume of plasma removed). Lipemia is caused by what? - correct answer ✅Increased dietary triglycerides Lipemia should be suspected if? - correct answer ✅HCT and Hemoglobin results don't match (higher than expected hemoglobin when compared to the HCT). What is another name for hematocrit (HCT)? - correct answer ✅Packed Cell Volume (PCV) Define the HCT? - correct answer ✅The % of RBCs in a volume of whole blood.
Describe the manual microhematocrit method. - correct answer ✅Centrifuge two capillary tubes at 10,000 RPMs for 5 minutes and take the average of the two tubes. How is HCT calculated using the automated method? - correct answer ✅HCT is calcuated using the MCV and RBC What are the RBC indices? - correct answer ✅* MCV (Mean corpuscular volume)
A peripheral blood smear with a MCHC of less than 32% will be? - correct answer ✅Hypochromatic A peripheral blood smear with a MCHC of 32-36% will be? - correct answer ✅Normochromatic A peripheral blood smear with a MCV of greater than 36% will be? - correct answer ✅Hyperchromatic A peripheral blood smear with a RDW of 12-15 will be? - correct answer ✅Normal A peripheral blood smear with a RDW of greater than 15 will be? - correct answer ✅Anisocytosis